NDLI: In vivo assembly of an archaeal virus studied with whole-cell electron cryotomography.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fu, Chi-Yu Wang, Kang Gan, Lu Lanman, Jason Khayat, Reza Young, Mark J. Jensen, Grant J. Doerschuk, Peter C. Johnson, John E.
Description	Country affiliation: United States Author Affiliation: Fu CY ( Department of Molecular Biology, The Scripps Research Institute, La Jolla, CA 92037, USA.)
Abstract	We applied whole-cell electron cryotomography to the archaeon Sulfolobus infected by Sulfolobus turreted icosahedral virus (STIV), which belongs to the PRD1-Adeno lineage of dsDNA viruses. STIV infection induced the formation of pyramid-like protrusions with sharply defined facets on the cell surface. They had a thicker cross-section than the cytoplasmic membrane and did not contain an exterior surface protein layer (S-layer). Intrapyramidal bodies often occupied the volume of the pyramids. Mature virions, procapsids without genome cores, and partially assembled particles were identified, suggesting that the capsid and inner membrane coassemble in the cytoplasm to form a procapsid. A two-class reconstruction using a maximum likelihood algorithm demonstrated that no dramatic capsid transformation occurred upon DNA packaging. Virions tended to form tightly packed clusters or quasicrystalline arrays while procapsids mostly scattered outside or on the edges of the clusters. The study revealed vivid images of STIV assembly, maturation, and particle distribution in cell.
File Format	HTM / HTML
ISSN	09692126
e-ISSN	18784186
DOI	10.1016/j.str.2010.10.005
Journal	Structure
Issue Number	12
Volume Number	18
Language	English
Publisher	Elsevier
Publisher Date	2010-12-08
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Molecular Biology Discipline Biochemistry Discipline Biotechnology Archaeal Viruses Physiology Cryoelectron Microscopy Electron Microscope Tomography Virus Assembly Chemistry Ultrastructure Capsid Proteins Metabolism Computer Simulation Likelihood Functions Models, Biological Sulfolobus Virology Tissue Distribution Viral Proteins Virion Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't Research Support, U.s. Gov't, Non-p.h.s.
Content Type	Text
Resource Type	Article
Subject	Structural Biology Molecular Biology

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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