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Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium.

Year: 2013 Volume: 12 Issue: 4

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Year: 2013 Volume: 12 Issue: 2

Year: 2013 Volume: 12 Issue: 1

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Visualization of mitochondria in living cells with a genetically encoded yellow fluorescent protein originating from a yellow-emitting luminous bacterium.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Karatani, Hajime Namikawa, Yuki Mori, Naomi Nishikawa, Yukihiro Imai, Saki Ihara, Yutaka Kinoshita, Aya Kitadokoro, Kengo Oyama, Hiroshi
Description	Country affiliation: Japan Author Affiliation: Karatani H ( Department of Biomolecular Engineering, Graduate School of Science and Technology, Kyoto Institute of Technology, 1 Hashigami-cho, Matsugasaki, Sakyo-ku, Kyoto 606-8585, Japan. karatani@kit.ac.jp)
Abstract	We have visualized redox and structural changes in the mitochondria of yeast Saccharomyces cerevisiae as a eukaryotic cell model using a genetically encoded yellow fluorescent protein (Y1-Yellow) and conventional fluorescence microscopy. Y1-Yellow originating from a yellow emitting luminous bacterium Aliivibrio sifiae Y1 was fused with a mitochondria-targeted sequence (mt-sequence). Y1-Yellow fluorescence arising only from the mitochondrial site and the color of yellow fluorescence could be easily differentiated from cellular autofluorescence and from that of conventional probes. Y1-Yellow expressing S. cerevisiae made the yellow fluorescence conspicuous at the mitochondrial site in response to reactive oxygen species (ROS) transiently derived in the wake of pretreatment with hydrogen peroxide. Based on our observation with Y1-Yellow fluorescence, we also showed that mitochondria rearrange to form a cluster structure surrounding chromosomal DNA via respiratory inhibition by cyanide, followed by the generation of ROS. In contrast, uptake of an uncoupler of oxidative phosphorylation is not responsible for mitochondrial rearrangement. These results indicate the utility of Y1-Yellow for visualization of mitochondrial vitality and morphology in living cells.
File Format	HTM / HTML
ISSN	1474905X
Issue Number	5
Volume Number	12
e-ISSN	14749092
Journal	Photochemical & Photobiological Sciences
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2013-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Chemistry Discipline Biology Aliivibrio Metabolism Bacterial Proteins Luminescent Proteins Mitochondria Chemistry Genetics Cyanides Toxicity Glucose Pharmacology Hydrogen Peroxide Indoles Microscopy, Fluorescence Reactive Oxygen Species Recombinant Proteins Biosynthesis Saccharomyces Cerevisiae Drug Effects Time-lapse Imaging Xanthenes Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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