NDLI: Bioluminescence of a firefly pupa: involvement of a luciferase isotype in the dim glow of pupae and eggs in the Japanese firefly, Luciola lateralis.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Oba, Yuichi Furuhashi, Mana Bessho, Manabu Sagawa, Shingo Ikeya, Haruyoshi Inouye, Satoshi
Spatial Coverage	Japan
Description	Country affiliation: Japan Author Affiliation: Oba Y ( Graduate School of Bioagricultural Sciences, Nagoya University, Chikusa-ku, Nagoya 464-8601, Japan. oba@agr.nagoya-u.ac.jp)
Abstract	We isolated cDNA for a luciferase isotype, LlLuc2, from the ovary of the Japanese firefly, Luciola lateralis. The gene product LlLuc2 showed 59% amino acid identity with LlLuc1, which had been isolated from the adult L. lateralis lantern. Molecular phylogenetic analysis indicated that LlLuc2 is an orthologue of LcLuc2 from Luciola cruciata. The spectral maxima of the luminescence by recombinant LlLuc1 and LlLuc2 were 550 and 539 nm, respectively. Quantitative PCR analysis revealed that LlLuc1 was expressed predominantly in larvae and adults, and LlLuc2 was expressed in eggs and pupae, which glow dimly, and we found that the in vivo luminescence spectra of the egg and pupa in L. lateralis were in good agreement with the in vitro luminescence spectrum by LlLuc2. These results suggest that, in L. lateralis, LlLuc1 is responsible for the yellowish luminescence of larval and adult lanterns, and LlLuc2 is responsible for the dim, greenish glow of eggs and whole pupae. Similar results were obtained in L. cruciata.
File Format	HTM / HTML
ISSN	1474905X
Issue Number	5
Volume Number	12
e-ISSN	14749092
Journal	Photochemical & Photobiological Sciences
Language	English
Publisher	Royal Society of Chemistry
Publisher Date	2013-05-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Chemistry Discipline Biology Luciferases Metabolism Amino Acid Sequence Animals Fireflies Classification Growth & Development Hydrogen-ion Concentration Japan Kinetics Larva Chemistry Genetics Molecular Sequence Data Ovum Phylogeny Recombinant Proteins Biosynthesis Journal Article
Content Type	Text
Resource Type	Article
Subject	Physical and Theoretical Chemistry

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Bioluminescence of a firefly pupa: involvement of a luciferase isotype in the dim glow of pupae and eggs in the Japanese firefly, Luciola lateralis.

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