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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lukinavicius, Grazvydas Reymond, Luc D'Este, Elisa Masharina, Anastasiya Göttfert, Fabian Ta, Haisen Güther, Angelika Fournier, Mathias Rizzo, Stefano Waldmann, Herbert Blaukopf, Claudia Sommer, Christoph Gerlich, Daniel W. Arndt, Hans-Dieter Hell, Stefan W. Johnsson, Kai |
| Description | Author Affiliation: Lukinavicius G ( 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2].); Reymond L ( 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland. [3].); D'Este E ( Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.); Masharina A ( Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.); Göttfert F ( Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.); Ta H ( Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.); Güther A ( Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.); Fournier M ( Bioimaging and Optics Platform, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland.); Rizzo S ( Max Planck Institute of Molecular Physiology, Dortmund, Germany.); Waldmann H ( Max Planck Institute of Molecular Physiology, Dortmund, Germany.); Blaukopf C ( Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.); Sommer C ( Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.); Gerlich DW ( Institute of Molecular Biotechnology of the Austrian Academy of Sciences, Vienna, Austria.); Arndt HD ( Institute of Organic Chemistry and Macromolecular Chemistry, Friedrich Schiller University, Jena, Germany.); Hell SW ( Department of NanoBiophotonics, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.); Johnsson K ( 1] Institute of Chemical Sciences and Engineering, École Polytechnique Fédérale de Lausanne, Lausanne, Switzerland. [2] National Centre of Competence of Research in Chemical Biology, Lausanne, Switzerland.) |
| Abstract | We introduce far-red, fluorogenic probes that combine minimal cytotoxicity with excellent brightness and photostability for fluorescence imaging of actin and tubulin in living cells. Applied in stimulated emission depletion (STED) microscopy, they reveal the ninefold symmetry of the centrosome and the spatial organization of actin in the axon of cultured rat neurons with a resolution unprecedented for imaging cytoskeletal structures in living cells. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| Issue Number | 7 |
| Volume Number | 11 |
| e-ISSN | 15487105 |
| Journal | Nature Methods |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2014-07-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Actins Chemistry Cytoskeleton Ultrastructure Fluorescent Dyes Microscopy, Confocal Methods Tubulin Animals Axons Cells, Cultured Erythrocytes Female Hela Cells Humans Male Mice Neurons Cytology Rats Rhodamines Silicon Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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