NDLI: CRISPR transcriptional repression devices and layered circuits in mammalian cells.

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CRISPR transcriptional repression devices and layered circuits in mammalian cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kiani, Samira Beal, Jacob Ebrahimkhani, Mohammad R. Huh, Jin Hall, Richard N. Xie, Zhen Li, Yinqing Weiss, Ron
Description	Country affiliation: United States Author Affiliation: Kiani S ( 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.); Beal J ( Raytheon BBN Technologies, Cambridge, Massachusetts, USA.); Ebrahimkhani MR ( Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.); Huh J ( 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Institute for the BioCentury, Korea Advanced Institute of Science and Technology, Dae); Hall RN ( 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.); Xie Z ( 1] Bioinformatics Division, Center for Synthetic & Systems Biology, Tsinghua National Laboratory for Information Science and Technology, Beijing, China. [2] Department of Automation, Tsinghua University, Beijing, China.); Li Y ( Department of Electrical Engineering and Computer Science, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA.); Weiss R ( 1] Synthetic Biology Center, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [2] Department of Biological Engineering, Massachusetts Institute of Technology, Cambridge, Massachusetts, USA. [3] Department of Electrical Engineering and Computer Science, Massachusetts Institute of)
Abstract	A key obstacle to creating sophisticated genetic circuits has been the lack of scalable device libraries. Here we present a modular transcriptional repression architecture based on clustered regularly interspaced palindromic repeats (CRISPR) system and examine approaches for regulated expression of guide RNAs in human cells. Subsequently we demonstrate that CRISPR regulatory devices can be layered to create functional cascaded circuits, which provide a valuable toolbox for engineering purposes.
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.2969
Journal	Nature Methods
Issue Number	7
Volume Number	11
Language	English
Publisher	Nature Publishing Group
Publisher Date	2014-07-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Clustered Regularly Interspaced Short Palindromic Repeats Genetics Genetic Engineering Instrumentation Rna, Guide Biosynthesis Gene Expression Regulation Gene Targeting Hek293 Cells Promoter Regions, Genetic Rna Polymerase Ii Rna Polymerase Iii Metabolism Transcription, Genetic Drug Effects Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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