NDLI: Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging

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Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Berlin, Shai Carroll, Elizabeth C. Newman, Zachary L. Okada, Hitomi O. Quinn, Carson M. Kallman, Benjamin Rockwell, Nathan C. Martin, Shelley S. Lagarias, J. Clark Isacoff, Ehud Y.
Description	Country affiliation: United States Author Affiliation: Berlin S ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Carroll EC ( Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, USA.); Newman ZL ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Okada HO ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Quinn CM ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Kallman B ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Rockwell NC ( Department of Molecular and Cell Biology, University of California, Berkeley, Berkeley, California, USA.); Martin SS ( Helen Wills Neuroscience Institute, University of California, Berkeley, Berkeley, California, USA.); Lagarias JC ( Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, USA.); Isacoff EY ( Department of Molecular and Cellular Biology, University of California, Davis, Davis, California, USA.)
Abstract	Circuit mapping requires knowledge of both structural and functional connectivity between cells. Although optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable genetically encoded Ca(2+) indicators that combines attributes of high-contrast photolabeling with high-sensitivity Ca(2+) detection in a single-color protein sensor. We demonstrated in cultured neurons and in fruit fly and zebrafish larvae how single cells could be selected out of dense populations for visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is transferrable to other sensors based on circularly permutated GFP (cpGFP).
File Format	HTM / HTML
ISSN	15487091
e-ISSN	15487105
DOI	10.1038/nmeth.3480
Journal	Nature Methods
Issue Number	9
Volume Number	12
Language	English
Publisher	Nature Publishing Group
Publisher Date	2015-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Calcium Signaling Physiology Calcium Metabolism Luminescent Proteins Neurons Cytology Optogenetics Animals Cell Tracking Cells, Cultured Drosophila Genetics Microscopy, Fluorescence Protein Engineering Zebrafish Research Support, N.i.h., Extramural Research Support, U.s. Gov't, P.h.s.
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology Biotechnology

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Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging

Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

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Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging

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Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging

Simultaneous whole-animal 3D imaging of neuronal activity using light-field microscopy.

Measuring calcium dynamics in living cells with genetically encodable calcium indicators.

Miniaturized integration of a fluorescence microscope

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Short-term high-resolution imaging of developing hippocampal neurons in culture.

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Photoactivatable Genetically-Encoded Calcium Indicators for targeted neuronal imaging