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| Content Provider | PubMed Central |
|---|---|
| Author | Berlin, Shai Carroll, Elizabeth C. Newman, Zachary L. Okada, Hitomi O. Quinn, Carson M. Kallman, Benjamin Rockwell, Nathan C. Martin, Shelley S. Lagarias, J. Clark Isacoff, Ehud Y. |
| Abstract | Circuit mapping requires knowledge of both structural and functional connectivity between cells. While optical tools have been made to assess either the morphology and projections of neurons or their activity and functional connections, few probes integrate this information. We have generated a family of photoactivatable Genetically Encoded Ca2+ Indicators (pa-GECIs) that combines attributes of high-contrast photo-labeling with high-sensitivity Ca2+ detection in a single-color, protein-sensor. We demonstrate the utility of pa-GECIs in cultured neurons and in vivo in Drosophila and zebrafish larvae. We show how single cells can be selected out of dense populations for Golgi-like visualization of morphology and high signal-to-noise measurements of activity, synaptic transmission and connectivity. Our design strategy is readily transferrable to other sensors based on circularly permutated GFP (cpGFP). |
| Related Links | http://dx.doi.org/10.1038/nmeth.3480 |
| Ending Page | 858 |
| Page Count | 7 |
| Starting Page | 852 |
| File Format | |
| ISSN | 15487105 |
| e-ISSN | 15487105 |
| Journal | Nature methods |
| Issue Number | 9 |
| Volume Number | 12 |
| Language | English |
| Publisher Date | 2015-09-01 |
| Access Restriction | Open |
| Subject Keyword | Research in Higher Education |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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