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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Juette, Manuel F. Terry, Daniel S. Wasserman, Michael R. Altman, Roger B. Zhou, Zhou Zhao, Hong Blanchard, Scott C. |
| Description | Country affiliation: United States Author Affiliation: Juette MF ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Terry DS ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Wasserman MR ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Altman RB ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Zhou Z ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Zhao H ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.); Blanchard SC ( Department of Physiology and Biophysics, Weill-Cornell Medical College, New York, New York, USA.) |
| Abstract | Single-molecule fluorescence microscopy is uniquely suited for detecting transient molecular recognition events, yet achieving the time resolution and statistics needed to realize this potential has proven challenging. Here we present a single-molecule imaging and analysis platform using scientific complementary metal-oxide semiconductor (sCMOS) detectors that enables imaging of 15,000 individual molecules simultaneously at millisecond rates. This system enabled the detection of previously obscured processes relevant to the fidelity mechanism in protein synthesis. |
| File Format | HTM / HTML |
| ISSN | 15487091 |
| e-ISSN | 15487105 |
| DOI | 10.1038/nmeth.3769 |
| Journal | Nature Methods |
| Issue Number | 4 |
| Volume Number | 13 |
| Language | English |
| Publisher | Nature Publishing Group |
| Publisher Date | 2016-04-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Clinical Laboratory Techniques Image Processing, Computer-assisted Microscopy, Fluorescence Instrumentation Molecular Imaging Rna, Transfer Ultrastructure Ribosomes Algorithms Bacteria Fluorescence Resonance Energy Transfer Time Factors Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Molecular Biology Biotechnology |
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