NDLI: Multi high-throughput approach for highly selective identification of vaccine candidates: the Group A Streptococcus case.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bensi, Giuliano Mora, Marirosa Tuscano, Giovanna Biagini, Massimiliano Chiarot, Emiliano Bombaci, Mauro Capo, Sabrina Falugi, Fabiana Manetti, Andrea G. O. Donato, Paolo Swennen, Erwin Gallotta, Marilena Garibaldi, Manuela Pinto, Vittoria Chiappini, Nico Musser, James M. Janulczyk, Robert Mariani, Massimo Scarselli, Maria Telford, John L. Grifantini, Renata Norais, Nathalie Margarit, Immaculada Grandi, Guido
Description	Country affiliation: Italy Author Affiliation: Bensi G ( Novartis Vaccines and Diagnostics, Via Fiorentina 1, 53100 Siena, Italy.)
Abstract	We propose an experimental strategy for highly accurate selection of candidates for bacterial vaccines without using in vitro and/or in vivo protection assays. Starting from the observation that efficacious vaccines are constituted by conserved, surface-associated and/or secreted components, the strategy contemplates the parallel application of three high throughput technologies, i.e. mass spectrometry-based proteomics, protein array, and flow-cytometry analysis, to identify this category of proteins, and is based on the assumption that the antigens identified by all three technologies are the protective ones. When we tested this strategy for Group A Streptococcus, we selected a total of 40 proteins, of which only six identified by all three approaches. When the 40 proteins were tested in a mouse model, only six were found to be protective and five of these belonged to the group of antigens in common to the three technologies. Finally, a combination of three protective antigens conferred broad protection against a panel of four different Group A Streptococcus strains. This approach may find general application as an accelerated and highly accurate path to bacterial vaccine discovery.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M111.015693
Journal	Molecular & Cellular Proteomics
Issue Number	6
Volume Number	11
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2012-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Antigens, Bacterial Immunology Bacterial Vaccines Administration & Dosage Streptococcal Infections Prevention & Control Streptococcus Pyogenes Animals Antibodies, Bacterial Blood Genetics Metabolism Bacterial Outer Membrane Proteins Cluster Analysis Flow Cytometry Hemolysis Mice Pharyngitis Microbiology Protein Array Analysis Proteome Secretion Vaccination Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

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1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
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3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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