NDLI: On marathons and Sprints: an integrated quantitative proteomics and transcriptomics analysis of differences between slow and fast muscle fibers.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Drexler, Hannes C. A. Ruhs, Aaron Konzer, Anne Mendler, Luca Bruckskotten, Mark Looso, Mario Günther, Stefan Boettger, Thomas Krüger, Marcus Braun, Thomas
Description	Country affiliation: Germany Author Affiliation: Drexler HC ( Max Planck Institute for Molecular Biomedicine, Röntgenstr 20, D-48149 Münster, Germany.)
Abstract	Skeletal muscle tissue contains slow as well as fast twitch muscle fibers that possess different metabolic and contractile properties. Although the distribution of individual proteins in fast and slow fibers has been investigated extensively, a comprehensive proteomic analysis, which is key for any systems biology approach to muscle tissues, is missing. Here, we compared the global protein levels and gene expression profiles of the predominantly slow soleus and fast extensor digitorum longus muscles using the principle of in vivo stable isotope labeling with amino acids based on a fully lysine-6 labeled SILAC-mouse. We identified 551 proteins with significant quantitative differences between slow soleus and fast extensor digitorum longus fibers out of >2000 quantified proteins, which greatly extends the repertoire of proteins differentially regulated between both muscle types. Most of the differentially regulated proteins mediate cellular contraction, ion homeostasis, glycolysis, and oxidation, which reflect the major functional differences between both muscle types. Comparison of proteomics and transcriptomics data uncovered the existence of fiber-type specific posttranscriptional regulatory mechanisms resulting in differential accumulation of Myosin-8 and -protein kinase 3 proteins and mRNAs among others. Phosphoproteome analysis of soleus and extensor digitorum longus muscles identified 2573 phosphosites on 973 proteins including 1040 novel phosphosites. The in vivo stable isotope labeling with amino acids-mouse approach used in our study provides a comprehensive view into the protein networks that direct fiber-type specific functions and allows a detailed dissection of the molecular composition of slow and fast muscle tissues with unprecedented resolution.
File Format	HTM / HTML
ISSN	15359476
e-ISSN	15359484
DOI	10.1074/mcp.M111.010801
Journal	Molecular & Cellular Proteomics
Issue Number	6
Volume Number	11
Language	English
Publisher	American Society for Biochemistry and Molecular Biology
Publisher Date	2012-06-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Proteomics Muscle Fibers, Fast-twitch Metabolism Muscle Fibers, Slow-twitch Muscle Proteins Proteome Transcriptome Amino Acid Sequence Animals Gene Expression Profiling Gene Expression Regulation Mice Mice, Inbred C57bl Molecular Sequence Data Molecular Weight Chemistry Genetics Oligonucleotide Array Sequence Analysis Peptide Fragments Phosphorylation Protein Processing, Post-translational Proteomics Sarcoplasmic Reticulum Calcium-transporting Atpases Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Analytical Chemistry Molecular Biology Biochemistry

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Differential regulation of the fiber type-specific gene expression of the sarcoplasmic reticulum calcium-ATPase isoforms induced by exercise training.

ATP Consumption by SR $Ca^{2+}$ Pumps Accounts for 50% of Resting Metabolic Rate in Mouse Fast and Slow Twitch Skeletal Muscle

On Marathons and Sprints: An Integrated Quantitative Proteomics and Transcriptomics Analysis of Differences Between Slow and Fast Muscle Fibers*

COPD elicits remodeling of the diaphragm and vastus lateralis muscles in humans.

$Ca^{2+}$ transport and heat production in vesicles derived from the sarcoplasmic reticulum terminal cisternae: Regulation by $K^{+}☆$

Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum $Ca^{2+}-ATPase$ assists in catalysis of $E_{2}/E_{2}P$ partial reactions

Muscle-specific deletion of exons 2 and 3 of the IL15RA gene in mice: effects on contractile properties of fast and slow muscles.

Myosin Heavy Chain Expression in Mouse Extraocular Muscle: More Complex Than Expected

On marathons and Sprints: an integrated quantitative proteomics and transcriptomics analysis of differences between slow and fast muscle fibers.

Similar Documents

Differential regulation of the fiber type-specific gene expression of the sarcoplasmic reticulum calcium-ATPase isoforms induced by exercise training.

ATP Consumption by SR $Ca^{2+}$ Pumps Accounts for 50% of Resting Metabolic Rate in Mouse Fast and Slow Twitch Skeletal Muscle

On Marathons and Sprints: An Integrated Quantitative Proteomics and Transcriptomics Analysis of Differences Between Slow and Fast Muscle Fibers*

COPD elicits remodeling of the diaphragm and vastus lateralis muscles in humans.

$Ca^{2+}$ transport and heat production in vesicles derived from the sarcoplasmic reticulum terminal cisternae: Regulation by $K^{+}☆$

Glutamate-183 in the conserved TGES motif of domain A of sarcoplasmic reticulum $Ca^{2+}-ATPase$ assists in catalysis of $E_{2}/E_{2}P$ partial reactions

Muscle-specific deletion of exons 2 and 3 of the IL15RA gene in mice: effects on contractile properties of fast and slow muscles.

Myosin Heavy Chain Expression in Mouse Extraocular Muscle: More Complex Than Expected

On marathons and Sprints: an integrated quantitative proteomics and transcriptomics analysis of differences between slow and fast muscle fibers.