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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Walzer, Mathias Qi, Da Mayer, Gerhard Uszkoreit, Julian Eisenacher, Martin Sachsenberg, Timo Gonzalez-Galarza, Faviel F. Fan, Jun Bessant, Conrad Deutsch, Eric W. Reisinger, Florian Vizcaíno, Juan Antonio Medina-Aunon, J. Alberto Albar, Juan Pablo Kohlbacher, Oliver Jones, Andrew R. |
| Description | Country affiliation: Germany Author Affiliation: Walzer M ( Quantitative Biology Center and Department of Computer Science, Center for Bioinformatics, University of Tübingen, Sand 14, 72076 Tübingen, Germany.) |
| Abstract | The range of heterogeneous approaches available for quantifying protein abundance via mass spectrometry (MS)(1) leads to considerable challenges in modeling, archiving, exchanging, or submitting experimental data sets as supplemental material to journals. To date, there has been no widely accepted format for capturing the evidence trail of how quantitative analysis has been performed by software, for transferring data between software packages, or for submitting to public databases. In the context of the Proteomics Standards Initiative, we have developed the mzQuantML data standard. The standard can represent quantitative data about regions in two-dimensional retention time versus mass/charge space (called features), peptides, and proteins and protein groups (where there is ambiguity regarding peptide-to-protein inference), and it offers limited support for small molecule (metabolomic) data. The format has structures for representing replicate MS runs, grouping of replicates (for example, as study variables), and capturing the parameters used by software packages to arrive at these values. The format has the capability to reference other standards such as mzML and mzIdentML, and thus the evidence trail for the MS workflow as a whole can now be described. Several software implementations are available, and we encourage other bioinformatics groups to use mzQuantML as an input, internal, or output format for quantitative software and for structuring local repositories. All project resources are available in the public domain from the HUPO Proteomics Standards Initiative http://www.psidev.info/mzquantml. |
| File Format | HTM / HTML |
| ISSN | 15359476 |
| e-ISSN | 15359484 |
| DOI | 10.1074/mcp.O113.028506 |
| Journal | Molecular & Cellular Proteomics |
| Issue Number | 8 |
| Volume Number | 12 |
| Language | English |
| Publisher | American Society for Biochemistry and Molecular Biology |
| Publisher Date | 2013-08-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Proteomics Mass Spectrometry Proteomics Databases, Protein Models, Theoretical Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Molecular Biology Biochemistry |
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