NDLI: Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry.

Please wait, while we are loading the content...

Comprehensive screening and selection of okra (Abelmoschus esculentus) germplasm for salinity tolerance at the seedling stage and during plant ontogeny

Development of a class-specific polyclonal antibody-based indirect competitive ELISA for detecting fluoroquinolone residues in milk.

Sphingosine 1-phosphate acts as an activator for the porcine Gpr3 of constitutively active G protein-coupled receptors

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry.

Optimization of enzymatic hydrolysis of cassava to obtain fermentable sugars.

Year: 2012 Volume: 13 Issue: 6

Year: 2012 Volume: 13 Issue: 5

Year: 2012 Volume: 13 Issue: 4

Year: 2012 Volume: 13 Issue: 3

Year: 2012 Volume: 13 Issue: 2

Year: 2012 Volume: 13 Issue: 1

Year: 2011 Volume: 12

Year: 2010 Volume: 11

Year: 2009 Volume: 10

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Xiao, Ying-ping Wu, Tian-xing Hong, Qi-hua Sun, Jiang-ming Chen, An-guo Yang, Cai-mei Li, Xiao-yan
Description	Country affiliation: China Author Affiliation: Xiao YP ( College of Animal Sciences, Zhejiang University, Hangzhou, China.)
Abstract	A novel metabolomic method based on gas chromatography/mass spectrometry (GC-MS) was applied to determine the metabolites in the serum of piglets in response to weaning and dietary L-glutamine (Gln) supplementation. Thirty-six 21-d-old piglets were randomly assigned into three groups. One group continued to suckle from the sows (suckling group), whereas the other two groups were weaned and their diets were supplemented with 1% (w/w) Gln or isonitrogenous L-alanine, respectively, representing Gln group or control group. Serum samples were collected to characterize metabolites after a 7-d treatment. Results showed that twenty metabolites were down-regulated significantly (P<0.05) in control piglets compared with suckling ones. These data demonstrated that early weaning causes a wide range of metabolic changes across arginine and proline metabolism, aminosugar and nucleotide metabolism, galactose metabolism, glycerophospholipid metabolism, biosynthesis of unsaturated fatty acid, and fatty acid metabolism. Dietary Gln supplementation increased the levels of creatinine, D-xylose, 2-hydroxybutyric acid, palmitelaidic acid, and -L-galactofuranose (P<0.05) in early weaned piglets, and were involved in the arginine and proline metabolism, carbohydrate metabolism, and fatty acid metabolism. A leave-one-out cross-validation of random forest analysis indicated that creatinine was the most important metabolite among the three groups. Notably, the concentration of creatinine in control piglets was decreased (P=0.00001) compared to the suckling piglets, and increased (P=0.0003) in Gln-supplemented piglets. A correlation network for weaned and suckling piglets revealed that early weaning changed the metabolic pathways, leading to the abnormality of carbohydrate metabolism, amino acid metabolism, and lipid metabolism, which could be partially improved by dietary Gln supplementation. These findings provide fresh insight into the complex metabolic changes in response to early weaning and dietary Gln supplementation in piglets.
File Format	HTM / HTML
ISSN	16731581
e-ISSN	18621783
DOI	10.1631/jzus.B1100343
Journal	Journal of Zhejiang University SCIENCE B
Issue Number	7
Volume Number	13
Language	English
Publisher	Springer
Publisher Date	2012-07-01
Publisher Place	China
Access Restriction	Open
Subject Keyword	Discipline Biomedicine Discipline Biotechnology Dietary Supplements Gas Chromatography-mass Spectrometry Glutamine Administration & Dosage Metabolome Physiology Milk, Human Metabolism Proteome Administration, Oral Animals Breast Feeding Swine Comparative Study Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Veterinary Medicine Biochemistry, Genetics and Molecular Biology Pharmacology, Toxicology and Pharmaceutics

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Response to weaning and dietary l-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry*

Effects of oral administration of heat-killed Enterococcus faecium strain NHRD IHARA in post-weaning piglets.

Plasma metabolomic profiling of dairy cows affected with ketosis using gas chromatography/mass spectrometry

Oral feeding with L-Glutamine and Nucleotides: impact on some GALT (gut associated lymphoid tissue) parameters and cell proliferation/death rates in weaning piglets

Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis, growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli.

Effects of the dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries.

Effect of maternal folic acid supplementation on hepatic proteome in newborn piglets.

Metabolomics and proteomics insights into hepatic responses of weaned piglets to dietary Spirulina inclusion and lysozyme supplementation

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry.

Similar Documents

Response to weaning and dietary l-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry*

Effects of oral administration of heat-killed Enterococcus faecium strain NHRD IHARA in post-weaning piglets.

Plasma metabolomic profiling of dairy cows affected with ketosis using gas chromatography/mass spectrometry

Oral feeding with L-Glutamine and Nucleotides: impact on some GALT (gut associated lymphoid tissue) parameters and cell proliferation/death rates in weaning piglets

Effects of dietary supplementation of lipid-encapsulated zinc oxide on colibacillosis, growth and intestinal morphology in weaned piglets challenged with enterotoxigenic Escherichia coli.

Effects of the dietary supplementation with fructooligosaccharides on the excretion of nitrogen and phosphorus in Miichthys miiuy fries.

Effect of maternal folic acid supplementation on hepatic proteome in newborn piglets.

Metabolomics and proteomics insights into hepatic responses of weaned piglets to dietary Spirulina inclusion and lysozyme supplementation

Response to weaning and dietary L-glutamine supplementation: metabolomic analysis in piglets by gas chromatography/mass spectrometry.