NDLI: Molecular and immunological characterization of a novel 32-kDa secreted protein of Babesia microti.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ooka, Hideo Terkawi, Mohamad A. Cao, Shinuo Aboge, Gabriel Goo, Youn-Kyoung Luo, Yuzi Li, Yan Nishikawa, Yoshifumi Igarashi, Ikuo Xuan, Xuenan
Description	Country affiliation: Japan Author Affiliation: Ooka H ( National Research Center for Protozoan Diseases, Obihiro University of Agriculture and Veterinary Medicine, Obihiro, Hokkaido 080-8555, Japan.)
Abstract	A cDNA encoding the Babesia microti 32-kDa protein was identified by serological immunoscreening of a cDNA expression library and designated as BmP32. The full length of BmP32 contains an open reading frame of 918 base pairs consisting of 306 amino acids having a significant homology with B. microti secreted antigen 1. Antiserum raised against recombinant protein (rBmP32) specifically reacted with a 32-kDa native protein of the parasite lysate using western blot analysis. The indirect immunofluorescent antibody test showed a preferable localization of BmP32 in the cytoplasm of the intra- and extracellular parasites. Moreover, BmP32 was secreted in the cytosol of infected erythrocytes, especially during the peak parasitemia and the recovery phase of the infection. Next, the antigenicity of rBmP32 was examined by an enzyme-linked immunosorbent assay (ELISA) and sera from mice experimentally infected with either B. microti or closely related parasites. ELISA was highly specific and sensitive when used for the detection of B. microti antibody in a mouse model. Furthermore, mice immunized with rBmP32 emulsified with Freund's adjuvant were not significantly protected against challenge infection with B. microt i. However, high antibody titer was detected just before the challenge infection. Our data suggest that rBmP32 may be a specific diagnostic antigen but not a subunit vaccine.
File Format	HTM / HTML
ISSN	00223395
Issue Number	5
Volume Number	98
e-ISSN	19372345
Journal	Journal of Parasitology
Language	English
Publisher	American Society of Parasitologists
Publisher Date	2012-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Parasitology Babesia Microti Chemistry Protozoan Proteins Genetics Immunology Animals Babesiosis Diagnosis Parasitology Blotting, Western Dna, Complementary Dna, Protozoan Electrophoresis, Polyacrylamide Gel Enzyme-linked Immunosorbent Assay Immune Sera Mice Molecular Weight Recombinant Proteins Sensitivity And Specificity Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Parasitology Ecology, Evolution, Behavior and Systematics

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