NDLI: Obtaining an isolate of Ancylostoma braziliense from dogs without the need for necropsy.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Liotta, Janice L. Lee, Alice C. Y. Aksel, Sarp Alkhalife, Ibrahim Cruz-Reyes, Alejandro Youn, HeeJeong Bienhoff, Stephen E. Bowman, Dwight D.
Description	Country affiliation: United States Author Affiliation: Liotta JL ( Department of Microbiology and Immunology, College of Veterinary Medicine, Cornell University, Ithaca, New York 14853, USA.)
Abstract	Isolation of a specific Ancylostoma species typically requires death of the source animal, or holding an animal long enough to collect feces after treatment, for worm recovery and identification. The reason for collecting worms is that the eggs are not easy to distinguish morphologically. In keeping with the 3 Rs of laboratory animal research (reduction, refinement, replacement), the objective of this study was to obtain an isolate of Ancylostoma braziliense from 1-time field-collected samples of canine feces without the need for killing the host. During a collection trip to Florida, fecal samples (n â=â 148) were collected and identified as containing eggs of Ancylostoma species (n â=â 64) using centrifugal sugar flotation. Eggs from hookworm-positive slides were washed into tubes, DNA was extracted, and 2 samples were identified as A. braziliense using restriction fragment length polymorphism (RFLP) with Hinf1. Larval cultures were initiated from these samples, and larvae from the cultures were returned to New York and used to inoculate a purpose-bred kitten with the goal of inhibiting the growth of any contaminating Ancylostoma caninum that might be present in the culture. The infection was patent at 15 days, and eggs were identified as A. braziliense by RFLP and DNA sequencing. Using forceps during endoscopy, 2 adult worms (1 male, 1 female) were recovered from the cat and identified morphologically as A. braziliense . Larvae were cultured from the feces of this cat and used to infect a laboratory-reared beagle dog. Additionally, worms recovered from the feces of the cat post-treatment were confirmed to be A. braziliense , except for 1 female A. caninum containing infertile eggs. The dog (patent 14 days post-infection) was also infected with A. braziliense as determined by RFLP and DNA sequencing of eggs and cultured larvae. Both the cat and dog were treated, verified to be no longer shedding eggs, and then placed into adoptive homes.
File Format	HTM / HTML
ISSN	00223395
Issue Number	5
Volume Number	98
e-ISSN	19372345
Journal	Journal of Parasitology
Language	English
Publisher	American Society of Parasitologists
Publisher Date	2012-10-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Parasitology Ancylostoma Isolation & Purification Ancylostomiasis Veterinary Dog Diseases Parasitology Anatomy & Histology Classification Animals Cat Diseases Cats Dogs Endoscopy Feces Female Male Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Parasitology Ecology, Evolution, Behavior and Systematics

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