| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Ku, Chai Siah Kim, Bohkyung Pham, Tho X. Yang, Yue Weller, Curtis L. Carr, Timothy P. Park, Young-Ki Lee, Ji-Young |
| Description |
Country affiliation: United States Author Affiliation: Ku CS ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .); Kim B ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .); Pham TX ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .); Yang Y ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .); Weller CL ( 2 Department of Biological Systems Engineering, University of Nebraska , Lincoln, Nebraska, USA .); Carr TP ( 3 Department of Nutrition and Health Sciences, University of Nebraska , Lincoln, Nebraska, USA .); Park YK ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .); Lee JY ( 1 Department of Nutritional Sciences, University of Connecticut , Storrs, Connecticut, USA .) |
| Abstract |
We previously demonstrated that Nostoc commune var. sphaeroids Kützing (NO), a blue-green alga (BGA), exerts a hypolipidemic effect in vivo and its lipid extract regulates the expression of genes involved in cholesterol and lipid metabolism in vitro. The objective of this study was to investigate whether the hypolipidemic effect of NO is attributed to an algal lipid or a delipidated fraction in vivo compared with Spirulina platensis (SP). Male C57BL/6J mice were fed an AIN-93M diet containing 2.5% or 5% of BGA (w/w) or a lipid extract equivalent to 5% of BGA for 4 weeks to measure plasma and liver lipids, hepatic gene expression, intestinal cholesterol absorption, and fecal sterol excretion. Plasma total cholesterol (TC) was significantly lower in 2.5% and 5% NO-fed groups, while plasma triglyceride (TG) levels were decreased in the 5% NO group compared with controls. However, neither NO organic extract (NOE) nor SP-fed groups altered plasma lipids. Hepatic mRNA levels of sterol regulatory element-binding protein 2, 3-hydroxy-3-methyl-glutaryl-CoA reductase (HMGR), carnitine palmitoyltransferase-1 , and acyl-CoA oxidase 1 were induced in 5% NO-fed mice, while there were no significant changes in hepatic lipogenic gene expression between groups. NO, but not NOE and SP groups, significantly decreased intestinal cholesterol absorption. When HepG2 cells and primary mouse hepatocytes were incubated with NOE and SP organic extract (SPE), there were marked decreases in protein levels of HMGR, low-density lipoprotein receptor, and fatty acid synthase. In conclusion, the nonlipid fraction of NO exerts TC and TG-lowering effects primarily by inhibiting intestinal cholesterol absorption and by increasing hepatic fatty acid oxidation, respectively. |
| File Format |
HTM / HTML |
| ISSN |
1096620X |
| Issue Number |
11 |
| Volume Number |
18 |
| e-ISSN |
15577600 |
| Journal |
Journal of Medicinal Food |
| Language |
English |
| Publisher |
Mary Ann Liebert, Inc. |
| Publisher Date |
2015-11-01 |
| Publisher Place |
United States |
| Access Restriction |
Subscribed |
| Subject Keyword |
Discipline Nutritional Sciences Biological Products Pharmacology Cholesterol Metabolism Hypolipidemic Agents Intestinal Absorption Drug Effects Lipids Liver Nostoc Commune Acyl Coenzyme A Animals Carnitine O-palmitoyltransferase Blood Dietary Supplements Fatty Acid Synthases Hep G2 Cells Humans Lipid Metabolism Genetics Lipoproteins, Ldl Male Mice, Inbred C57bl Chemistry Plant Extracts Rna, Messenger Receptors, Ldl Spirulina Triglycerides Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Nutrition and Dietetics Medicine |
