NDLI: ACE-I Inhibitory Activity from Phaseolus lunatus and Phaseolus vulgaris Peptide Fractions Obtained by Ultrafiltration.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Betancur-Ancona, David Dávila-Ortiz, Gloria Chel-Guerrero, Luis Antonio Torruco-Uco, Juan Gabriel
Description	Author Affiliation: Betancur-Ancona D ( 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México.); Dávila-Ortiz G ( 2 Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional , México, D.F., México.); Chel-Guerrero LA ( 1 Facultad de Ingeniería Química, Universidad Autónoma de Yucatán , Mérida, México.); Torruco-Uco JG ( 2 Escuela Nacional de Ciencias Biológicas, Instituto Politécnico Nacional , México, D.F., México.)
Abstract	The involvement of angiotensin-I-converting enzyme (ACE-I) as one of the mechanisms controlling blood pressure is being studied to find alternative means of control of hypertension on human beings. On the market there are synthetic drugs that can control it, but these can cause undesirable health side effects. In this work was assessed the fractionation by ultrafiltration of the Lima bean (Phaseolus lunatus) and Jamapa bean (Phaseolus vulgaris), protein hydrolysates obtained with Alcalase(®) and Flavourzyme(®) on ACE-I inhibitory activity. Four membranes of different molecular cutoffs (10, 5, 3, and 1 kDa) were used. Fractions that had a higher inhibitory activity in both legumes were denominated as E (<1 kDa) with IC50 of 30.3 and 51.8 µg/mL values for the P. lunatus with Alcalase and Flavourzyme, respectively, and for the Phaseolus vulgaris with Alcalase and Flavourzyme with about 63.8 and 65.8 µg/mL values, respectively. The amino acid composition of these fractions showed residues in essential amino acids, which make a good source of energy and amino acids. On the other hand, the presence of hydrophobic amino acids such as V and P is a determining factor in the ACE-I inhibitor effect. The results suggest the possibility of obtaining and utilizing these peptide fractions in the development and innovation of a functional product that helps with treatment and/or prevention of hypertension.
File Format	HTM / HTML
ISSN	1096620X
Issue Number	11
Volume Number	18
e-ISSN	15577600
Journal	Journal of Medicinal Food
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2015-11-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Nutritional Sciences Angiotensin-converting Enzyme Inhibitors Pharmacology Hypertension Metabolism Peptide Fragments Peptidyl-dipeptidase A Phaseolus Chemistry Plant Proteins Protein Hydrolysates Amino Acids Analysis Therapeutic Use Blood Pressure Drug Effects Humans Drug Therapy Physiopathology Inhibitory Concentration 50 Species Specificity Ultrafiltration Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Nutrition and Dietetics Medicine

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