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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Omiccioli, Enrica Amagliani, Giulia Brandi, Giorgio Magnani, Mauro |
| Description | Country affiliation: Italy Author Affiliation: Omiccioli E ( Dipartimento di Scienze Biomolecolari, Sez. di Biotecnologie, Università di Urbino Carlo Bo, via Campanella 1, 61032 Fano (PU), Italy. enrica.omiccioli@uniurb.it) |
| Abstract | Intoxications and infections caused by food-borne pathogens represent an increasing public health problem, and diagnostic tests in multiplex format are needed for the rapid identification of food contaminations caused by more than one microbial species. We have developed a multiple PCR-based platform for the simultaneous detection of the widespread milk-associated pathogens Salmonella spp., Listeria monocytogenes and Escherichia coli O157. The assay combines an enrichment step in a medium properly formulated for the simultaneous growth of target pathogens, a DNA isolation method, and a multiplex Real-Time PCR detection system based either on dual-labelled probes (mRT-PCR), or on melting curve analysis (mHRM). The second, producing a distinct peak for each amplification product, allows the qualitative assessment of pathogen presence. Moreover, the internal amplification control (IAC) included in the reaction, ensuring the reliability of results, complies with quality management programmes. Inclusivity and exclusivity were 100% each, with a detection limit of 1 CFU for each pathogen in a total of five 25 ml-aliquots of raw milk, and a duration of two working days. The assay represents an alternative approach for the qualitative detection of the cited bacterial species, suitable for a relatively inexpensive screening of several milk samples, reducing the turnaround time and the workload. |
| File Format | HTM / HTML |
| ISSN | 07400020 |
| Issue Number | 6 |
| Volume Number | 26 |
| e-ISSN | 10959998 |
| Journal | Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-09-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Nutritional Sciences Escherichia Coli O157 Isolation & Purification Food Contamination Analysis Listeria Monocytogenes Milk Microbiology Polymerase Chain Reaction Salmonella Animals Colony Count, Microbial Methods Dna, Bacterial Humans Sensitivity And Specificity Species Specificity Time Factors Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Microbiology Food Science |
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