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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Garrido, Alejandro Chapela, María-José Román, Belén Fajardo, Paula Vieites, Juan M. Cabado, Ana G. |
| Description | Country affiliation: Spain Author Affiliation: Garrido A ( Microbiology and Toxins Area, ANFACO-CECOPESCA, Campus Univ. 16, 36310 Vigo PO, Spain.) |
| Abstract | A wide variety of qPCR methods currently exist for Salmonella spp., Escherichia coli O157 and Listeria monocytogenes detection. These methods target several genes and use different detection chemistries, either in simplex or in multiplex formats. However, the majority of these methods have not been carefully validated, and the number of validated methods that use multiplex qPCR is even lower. The aim of the present study was to develop and validate a multiplex qPCR method from previously validated simplex qPCR primers and probes. A modified broth medium was selected and primary and secondary enrichment times were further optimized. Efficiency of the newly combined qPCR system was comprised between 91% and 108%, for simplex and multiplex analyses. A total of 152 food and environmental, natural and spiked samples, were analyzed for the evaluation of the method obtaining values above 91% that were reached for all the quality parameters analyzed. A very low limit of detection (5 cfu/25 g after enrichment) for simultaneous identification of these 3 pathogens was obtained. |
| File Format | HTM / HTML |
| ISSN | 01681605 |
| Issue Number | 1 |
| Volume Number | 164 |
| e-ISSN | 18793460 |
| Journal | International Journal of Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2013-06-03 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Dna Primers Escherichia Coli O157 Salmonella Research Support, Non-u.s. Gov't Discipline Microbiology Sensitivity And Specificity Real-time Polymerase Chain Reaction Listeria Monocytogenes Multiplex Polymerase Chain Reaction Food Microbiology Standards Journal Article Culture Media Transaminases Validation Studies Genetics Physiology Discipline Nutritional Sciences Carbohydrate Epimerases Methods |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Safety, Risk, Reliability and Quality Food Science |
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