NDLI: Amplified Fragment Length Polymorphism and Multi-Locus Sequence Typing for high-resolution genotyping of Listeria monocytogenes from foods and the environment.

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Year: 2009 Volume: 26

Year: 2008 Volume: 25

Amplified Fragment Length Polymorphism and Multi-Locus Sequence Typing for high-resolution genotyping of Listeria monocytogenes from foods and the environment.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Parisi, Antonio Latorre, Laura Normanno, Giovanni Miccolupo, Angela Fraccalvieri, Rosa Lorusso, Vanessa Santagada, Gianfranco
Description	Country affiliation: Italy Author Affiliation: Parisi A ( Experimental Zooprophylactic Institute of Apulia and Basilicata, Foggia, Italy. parisi.izs@tin.it)
Abstract	Standardized tools for typing Listeria monocytogenes isolates are required in epidemiological surveys investigating food-borne disease outbreaks and in the food-processing environment to identify the sources of contamination and routes by which the organisms are spread. In this survey Amplified Fragment Length Polymorphism (AFLP) and Multi-Locus Sequence Typing (MLST) have been used to study 103 L. monocytogenes isolates from food and environmental sources. A total of 62 AFLP types and 66 MLST Sequence Types were identified. AFLP and MLST produced similar results in terms of discriminating power. The Discrimination Index calculated for the two techniques was 0.976 for AFLP and 0.972 for MLST. These values were appreciably higher compared to serotyping (0.739). A good congruence was observed between AFLP and MLST. The present study demonstrated that AFLP and MLST subtyping are suitable tools for studying the epidemiology of L. monocytogenes. The great advantage of MLST over AFLP and other molecular typing methods based on fragment fingerprinting lies in the unambiguity of sequence data while AFLP is less costly and highly processive. In conclusion the two methods can be perfectly integrated for high-resolution genotyping of L. monocytogenes.
File Format	HTM / HTML
ISSN	07400020
Issue Number	1
Volume Number	27
e-ISSN	10959998
Journal	Food Microbiology
Language	English
Publisher	Elsevier
Publisher Date	2010-02-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Nutritional Sciences Bacterial Typing Techniques Methods Environmental Microbiology Food Microbiology Listeria Monocytogenes Genetics Isolation & Purification Amplified Fragment Length Polymorphism Analysis Genotype Classification Phylogeny Sequence Analysis, Dna Evaluation Studies Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Microbiology Food Science

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