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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Lomonaco, Sara Decastelli, Lucia Nucera, Daniele Gallina, Silvia Manila Bianchi, Daniela Civera, Tiziana |
| Description | Country affiliation: Italy Author Affiliation: Lomonaco S ( Dipartimento di Patologia Animale, Università degli Studi di Torino, Facoltà di Medicina Veterinaria, Via Leonardo da Vinci 44, 10095 Grugliasco, Torino, Italy.) |
| Abstract | L. monocytogenes represents a primary concern in the production of Gorgonzola, a Protected Designation of Origin (PDO) Italian blue-veined cheese produced only in the Piedmont and Lombardy regions. L. monocytogenes isolates (N=95) obtained from Gorgonzola rinds, paste, and production/ripening environments were serotyped and then genotyped using Pulsed Field Gel Electrophoresis (PFGE). The goal of this study was to investigate the variability of L. monocytogenes PFGE-types across different PDO Gorgonzola manufacturers (N=22). The majority of the strains (88%) were serotyped as 1/2a. PFGE identified 2 major pulse-types grouping 62 strains, detected from different plants and years, suggesting the presence of persistent and niche-adapted L. monocytogenes. In 9 plants, environmental strains shared the same pulse-types with strains from rinds or paste, suggesting a possible transmission pathway. Encouragingly, L. monocytogenes was retrieved from only 1 paste, indicating that production processes were under control in 21 plants. In the remaining plant, un-effective pasteurization or cross-contamination during production processes could be the cause of the contamination. Consequently, it is imperative that producers operate under the total respect of the Good Manufacturing Practices and following the principles of the Hazard Analysis Critical Control Point plans, in order to contain contamination throughout the whole processing. |
| File Format | HTM / HTML |
| ISSN | 01681605 |
| Issue Number | 3 |
| Volume Number | 128 |
| e-ISSN | 18793460 |
| Journal | International Journal of Food Microbiology |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2009-01-15 |
| Publisher Place | Netherlands |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Microbiology Food Contamination Consumer Product Safety Humans Isolation & Purification Listeria Monocytogenes Genotype Cheese Electrophoresis, Gel, Pulsed-field Phylogeny Food Microbiology Journal Article Growth & Development Phenotype Time Factors Analysis Bacterial Typing Techniques Classification Food Handling Discipline Nutritional Sciences Methods |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Microbiology Safety, Risk, Reliability and Quality Food Science |
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