NDLI: Novel ceramic bone replacement material Osbone® in a comparative in vitro study with osteoblasts.

Please wait, while we are loading the content...

Comparative study of the osseointegration of dental implants after different bone augmentation techniques: vascularized femur flap, non-vascularized femur graft and mandibular bone graft.

Novel ceramic bone replacement material Osbone® in a comparative in vitro study with osteoblasts.

A novel in vitro approach to assess the fit of implant frameworks.

Vertical palatal bone dimensions on lateral cephalometry and cone-beam computed tomography: implications for palatal implant placement.

Use of self-setting -tricalcium phosphate for maxillary sinus augmentation in rabbit.

Delayed osteoblast differentiation and altered inflammatory response around implants placed in incisor sockets of type 2 diabetic rats.

Benign paroxysmal positional vertigo following closed sinus floor elevation procedure: mallet osteotomes vs. screwable osteotomes. A triple blind randomized controlled trial.

Early bacterial colonization and soft tissue health around zirconia and titanium abutments: an in vivo study in man.

Influence of abutment material on the gingival color of implant-supported all-ceramic restorations: a prospective multicenter study.

Intra-oral bone harvesting: two methods compared using histological and histomorphometric assessments.

Influence of surface roughness and shape on microdamage of the osseous surface adjacent to titanium dental implants.

Long-term prosthetic aftercare of direct vs. indirect attachment incorporation techniques to mandibular implant-supported overdenture.

Chemical modification of an implant surface increases osteogenesis and simultaneously reduces osteoclastogenesis: an in vitro study.

Effects of growth hormone on initial bone formation around dental implants: a dog study.

Deflections of an implant-supported cantilever beam subjected to vertically directed loads. In vitro measurements in three dimensions using an optoelectronic method. II Analysis of methodological errors.

Year: 2011 Volume: 22 Issue: 5

Year: 2011 Volume: 22 Issue: 4

Year: 2011 Volume: 22 Issue: 3

Year: 2011 Volume: 22 Issue: 2

Year: 2011 Volume: 22 Issue: 1

Year: 2010 Volume: 21

Year: 2009 Volume: 20

Year: 2008 Volume: 19

Novel ceramic bone replacement material Osbone® in a comparative in vitro study with osteoblasts.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Bernhardt, Anne Lode, Anja Peters, Fabian Gelinsky, Michael
Description	Country affiliation: Germany Author Affiliation: Bernhardt A ( Max Bergmann Center of Biomaterials and Institute for Materials Science, Technische Universität Dresden, Dresden, Germany. abernhardt@nano.tu-dresden.de)
Abstract	OBJECTIVE: Hydroxyapatite (HA) is a very common ceramic material for bone replacement due to its similarity in composition to the mineral phase of natural bone. A recently developed bone graft material is Osbone(®), a synthetic HA ceramic available as porous granules with different sizes and block forms. The goal of this study was to characterise Osbone(®) in vitro in comparison to the already established calcium phosphate-based bone grafts Cerasorb M(®) and Bio-Oss(®). MATERIALS AND METHODS: Adhesion and proliferation of SaOS-2 osteoblasts were evaluated quantitatively by determining DNA content and lactate dehydrogenase (LDH) activity and qualitatively by scanning electron microscopy (SEM). In addition, MTT cell vitality staining was performed to confirm the attachment of viable cells to the different materials. Osteogenic differentiation of the cells was evaluated by means of alkaline phosphatase (ALP) activity quantification as well as by gene expression analysis of osteogenic markers using reverse transcriptase PCR. RESULTS: MTT staining after 1 day of adhesion showed viable cells on all examined materials. DNA content and LDH activity revealed proliferation of osteoblasts on Osbone(®) and Cerasorb M(®), but not on Bio-Oss(®) during cultivation over 28 days. SEM showed a well-spread morphology of cells attached to both Osbone(®) and Cerasorb M(®). We detected an increase of specific ALP activity during cultivation of osteoblasts on Osbone(®) and Cerasorb M(®) as well as expression of the bone-related genes ALP, osteonectin, osteopontin and bone sialoprotein II on both materials. CONCLUSIONS: Osbone(®) granules support proliferation and osteogenic differentiation in vitro and are therefore promising candidates for in vivo applications.
File Format	HTM / HTML
ISSN	09057161
Issue Number	6
Volume Number	22
e-ISSN	16000501
Journal	Clinical Oral Implants Research
Language	English
Publisher	Wiley-Blackwell
Publisher Date	2011-06-01
Publisher Place	Denmark
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Dentistry Discipline General Surgery Bone Substitutes Chemistry Ceramics Durapatite Osteoblasts Physiology Alkaline Phosphatase Analysis Calcium Phosphates Cell Adhesion Cell Differentiation Cell Line Cell Proliferation Cell Survival Coloring Agents Dna Humans Integrin-binding Sialoprotein L-lactate Dehydrogenase Materials Testing Microscopy, Electron, Scanning Minerals Osteogenesis Osteonectin Osteopontin Porosity Reverse Transcriptase Polymerase Chain Reaction Surface Properties Tetrazolium Salts Thiazoles Comparative Study Journal Article
Content Type	Text
Resource Type	Article
Subject	Oral Surgery

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Comparison of in vitro biocompatibility of NanoBone(®) and BioOss(®) for human osteoblasts.

In-vitro analysis of rhBMP-2 effects in human osteogenic cells.

Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.

Gene array of primary human osteoblasts exposed to enamel matrix derivative in combination with a natural bone mineral.

Potential induction of bone regeneration by nacre: an in vitro study.

A novel three-dimensional bone chip organ culture.

Novel ceramic bone replacement material CeraBall seeded with human mesenchymal stem cells.

Influence of nicotine on the biological activity of rabbit osteoblasts.

Effect of bioaggregate on mineral-associated gene expression in osteoblast cells.

Novel ceramic bone replacement material Osbone® in a comparative in vitro study with osteoblasts.

Similar Documents

Comparison of in vitro biocompatibility of NanoBone(®) and BioOss(®) for human osteoblasts.

In-vitro analysis of rhBMP-2 effects in human osteogenic cells.

Effects of the association between a calcium hydroxide paste and 0.4% chlorhexidine on the development of the osteogenic phenotype in vitro.

Gene array of primary human osteoblasts exposed to enamel matrix derivative in combination with a natural bone mineral.

Potential induction of bone regeneration by nacre: an in vitro study.

A novel three-dimensional bone chip organ culture.

Novel ceramic bone replacement material CeraBall seeded with human mesenchymal stem cells.

Influence of nicotine on the biological activity of rabbit osteoblasts.

Effect of bioaggregate on mineral-associated gene expression in osteoblast cells.

Novel ceramic bone replacement material Osbone® in a comparative in vitro study with osteoblasts.