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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | da Silva, Raquel Assed Bezerra Leonardo, Mário Roberto da Silva, Léa Assed Bezerra de Castro, Larissa Moreira Spinola Rosa, Adalberto Luiz de Oliveira, Paulo Tambasco |
| Description | Country affiliation: Brazil Author Affiliation: da Silva RA ( Department of Pediatric Clinic, Preventive and Social Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Ribeirão Preto, SP, Brazil. raquel@forp.usp.br) |
| Abstract | The present study aimed to evaluate whether the association between a calcium hydroxide paste (Calen paste) and 0.4% chlorhexidine (CHX) affects the development of the osteogenic phenotype in vitro. With rat calvarial osteogenic cell cultures, the following parameters were assayed: cell morphology and viability, alkaline phosphatase activity, total protein content, bone sialoprotein immunolocalization, and mineralized nodule formation. Comparisons were carried out by using the nonparametric Kruskal-Wallis test (level of significance, 5%). The results showed that the association between Calen paste and 0.4% CHX did not affect the development of the osteogenic phenotype. No significant changes were observed in terms of cell shape, cell viability, alkaline phosphatase activity, and the total amount of bone-like nodule formation among control, Calen, or Calen + CHX groups. The strategy to combine Ca(OH)(2) and CHX to promote a desirable synergistic antibacterial effect during endodontic treatment in vivo might not significantly affect osteoblastic cell biology. |
| File Format | HTM / HTML |
| ISSN | 00992399 |
| Issue Number | 12 |
| Volume Number | 34 |
| e-ISSN | 18783554 |
| Journal | Journal of Endodontics |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2008-12-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Cell Proliferation Rats, Wistar Organic Chemicals Coloring Agents Calcium Hydroxide Alkaline Phosphatase Osteoblasts Root Canal Irrigants Journal Article Proteins Tetrazolium Salts Sialoglycoproteins Cell Shape Thiazoles Chlorhexidine Bone Matrix Indoles Root Canal Filling Materials Discipline Dentistry Cell Survival Cells, Cultured Osteopontin Rats Pharmacology Drug Effects Animals Calcification, Physiologic Analysis Integrin-binding Sialoprotein Osteogenesis Drug Combinations Fluorescent Dyes |
| Content Type | Text |
| Resource Type | Article |
| Subject | Dentistry |
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