NDLI: Detection of lung cancer using MAGE A1-6 and SSX4 RT-PCR expression profiles in the bronchial wash fluid.

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An attempt for combining microarray data sets by adjusting gene expressions.

Detection of lung cancer using MAGE A1-6 and SSX4 RT-PCR expression profiles in the bronchial wash fluid.

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An Attempt for Combining Microarray Data Sets by Adjusting Gene Expressions

Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

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Detection of lung cancer using MAGE A1-6 and SSX4 RT-PCR expression profiles in the bronchial wash fluid.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lee, Kwan-Ho Shin, Kyung-Chul Lee, Chae-Hun Jheon, Sang-Hoon Jeon, Chang-Ho
Description	Author Affiliation: Lee KH ( Department of Internal Medicine, College of Medicine, Yeungnam University, Daegu, Korea.)
Abstract	PURPOSE: Bronchial wash fluid may be a useful for detecting lung cancer. To increase the detection rates, we performed molecular analysis with using MAGE A1-6 and SSX4 RT-PCR on bronchial wash fluid specimens. MATERIALS AND METHODS: We obtained 57 lung cancer tissue specimens by bronchoscopic biopsy and 131 bronchial washes from 96 patients with lung cancer and 35 patients with benign lung diseases. The MAGE A1-6 and SSX4 gene expressions were investigated in the cancer tissue specimens and bronchial wash fluids. We evaluated the positive detection rates of these methods according to the cytology results and the clinical findings. RESULTS: For the cancer tissue specimens and the bronchial wash fluid, the positive detection rate of MAGE or SSX4 was 91.2% and 75.0%, respectively. Combined MAGE and SSX4 PCR and cytology tests showed an 83.3% detection rate for the bronchial wash fluid. From bronchial washes of patients with benign lung diseases, the positive rates of using MAGE or SSX4 was 11.4%. In the bronchial wash fluid of lung cancer patients, 66.7% of the peripheral cancers were detected by MAGE or SSX4, while examination with cytology did not detect any peripheral lung cancer. CONCLUSION: The application of both MAGE and SSX4 showed high sensitivity and specificity for the detection of lung cancer. Thus, MAGE and SSX4 RT-PCR may be effectively utilized as additional methods to improve detection of lung cancer with using bronchial wash fluids.
File Format	HTM / HTML
ISSN	15982998
e-ISSN	20059256
DOI	10.4143/crt.2007.39.2.69
Journal	Cancer Research and Treatment
Issue Number	2
Volume Number	39
Language	English
Publisher	Korean Cancer Association.
Publisher Date	2007-06-01
Publisher Place	Korea (South)
Access Restriction	Open
Subject Keyword	Discipline Neoplasms
Content Type	Text
Resource Type	Article
Subject	Cancer Research Oncology

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Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

Detection of Lung Cancer using MAGE A1-6 and SSX4 RT-PCR Expression Profiles in the Bronchial Wash Fluid

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Detection of lung cancer using MAGE A1-6 and SSX4 RT-PCR expression profiles in the bronchial wash fluid.

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