| Content Provider |
World Health Organization (WHO)-Global Index Medicus |
| Author |
Miguel, Paulo Sérgio Balbino de Oliveira, Marcelo Nagem Valério Delvaux, Júlio César de Jesus, Guilherme Luiz Borges, Arnaldo Chaer Tótola, Marcos Rogério Neves, Júlio César Lima Costa, Maurício Dutra |
| Description |
Country affiliation: Brazil Author Affiliation: Miguel PS ( Departamento de Microbiologia/Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.); de Oliveira MN ( Departamento de Ciências Básicas da Vida, Universidade Federal de Juiz de Fora, Campus Governador Valadares, Juiz de Fora, MG, 36570-000, Brazil.); Delvaux JC ( Coordenação geral de Ensino e Extensão, Instituto Federal do Triângulo Mineiro, Ituiutaba, MG, 38035-200, Brazil.); de Jesus GL ( Celulose Nipo-Brasileira (CENIBRA), Belo Oriente, MG, 35196-000, Brazil.); Borges AC ( Departamento de Microbiologia/Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.); Tótola MR ( Departamento de Microbiologia/Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.); Neves JC ( Departamento de solos, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil.); Costa MD ( Departamento de Microbiologia/Instituto de Biotecnologia Aplicada à Agropecuária, Universidade Federal de Viçosa, Viçosa, MG, 36570-000, Brazil. madcosta@ufv.br.) |
| Abstract |
The relationships between plants and endophytic bacteria significantly contribute to plant health and yield. However, the microbial diversity in leaves of Eucalyptus spp. is still poorly characterized. Here, we investigated the endophytic diversity in leaves of hybrid Eucalyptus grandis x E. urophylla (Eucalyptus 'urograndis') by using culture-independent and culture-dependent approaches, to better understand their ecology in leaves at different stages of Eucalyptus development, including bacteria with N2 fixation potential. Firmicutes, Proteobacteria (classes alpha-, beta- and gamma-) and Actinobacteria were identified in the Eucalyptus 'urograndis' endophytic bacterial community. Within this community, the species Novosphingobium barchaimii, Rhizobium grahamii, Stenotrophomonas panacihumi, Paenibacillus terrigena, P. darwinianus and Terrabacter lapilli represent the first report these bacteria as endophytes. The diversity of the total endophytic bacteria was higher in the leaves from the 'field' (the Shannon-Wiener index, 2.99), followed by the indices obtained in the 'clonal garden' (2.78), the 'recently out from under shade (2.68), 'under shade' (2.63) and 'plants for dispatch' (2.51). In contrast, for diazotrophic bacteria, the highest means of these indices were obtained from the leaves of plants in the 'under shade' (2.56), 'recently out from under shade (2.52)' and 'field' stages (2.54). The distribution of the endophytic bacterial species in Eucalyptus was distinct and specific to the development stages under study, and many of the species had the potential for nitrogen fixation, raising the question of whether these bacteria could contribute to overall nitrogen metabolism of Eucalyptus. |
| File Format |
HTM / HTML |
| ISSN |
00036072 |
| Issue Number |
6 |
| Journal |
Antonie van Leeuwenhoek |
| Volume Number |
109 |
| e-ISSN |
15729699 |
| Language |
English |
| Publisher |
Springer |
| Publisher Date |
2016-06-01 |
| Publisher Place |
Netherlands |
| Access Restriction |
One Nation One Subscription (ONOS) |
| Subject Keyword |
Discipline Microbiology Discipline Immunology |
| Content Type |
Text |
| Resource Type |
Article |
| Subject |
Medicine Molecular Biology Microbiology |
