NDLI: Analysis of endophytic bacterial community composition by 16S rDNA clone library in Achnatherum inebrians

Content Provider	Springer Nature Link
Author	Shi, Ying Wu Zhang, Xuebing Lou, Kai
Copyright Year	2014
Abstract	The endophytic bacterial diversity of drunken horse grass (DHG) (Achnatherum inebrians) growing in native habitat station in Xinjiang Province, China was analyzed by 16S rDNA cloning, amplified ribosomal DNA restriction analysis (ARDRA), and sequence homology comparison. To effectively exclude the interference of chloroplast DNA and mitochondrial DNA of DHG, a pair of bacterial PCR primers was selected to specifically amplify bacterial 16S rDNA sequences directly from DHG root tissues. Among 249 positive clones in the 16S rDNA library of endophytes, 57 OTUs were identified based on the similarity of the ARDRA banding profiles. Sequence analysis revealed diverse phyla of bacteria in the 16S rDNA library, which consisted of alpha, beta, gamma, delta, and epsilon subclasses of the Proteobacteria, Flavobacteria, Actinobacteria, Sphingobacteria, and uncultured bacteria. The dominant group was Proteobacteria, whie the most numerous genus was Bacillus. More than 8.00% of the total clones showed a high similarity to uncultured bacteria, suggesting that nonculturable bacteria make a significant part of DHG endophytic bacterial community. To our knowledge, this is the first report that endophytic bacteria associated with DHG are revealed by the culture-independent approach. The results suggest that the diversity of endophytic bacteria is abundant in DHG.
Starting Page	888
Ending Page	895
Page Count	8
File Format	PDF
ISSN	00262617
Journal	Microbiology
Volume Number	83
Issue Number	6
e-ISSN	16083237
Language	English
Publisher	Pleiades Publishing
Publisher Date	2014-11-25
Publisher Place	Moscow
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	endophytic bacteria Achnatherum inebriants bacterial diversity 16S rDNA library Microbiology Medical Microbiology
Content Type	Text
Resource Type	Article
Subject	Microbiology Applied Microbiology and Biotechnology

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