NDLI: Picobirnavirus detection in bovine and buffalo calves from foothills of Himalaya and Central India.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Malik, Yashpal Singh Chandrashekar, K. M. Sharma, Kuldeep Haq, Adil A. Vaid, Nirupama Chakravarti, Somendu Batra, Munish Singh, Rashmi Pandey, A. B.
Spatial Coverage	India
Description	Country affiliation: India Author Affiliation: Malik YS ( Division of Virology, Indian Veterinary Research Institute, Mukteswar, Nainital, Uttarakhand 263138, India. malikyps@yahoo.com)
Abstract	The present study describes detection of picobirnavirus (PBV) in faecal samples from bovine and buffalo calves employing the polyacrylamide gel electrophoresis (PAGE). A total of 136 faecal samples from buffalo (n = 122) and cow calves (n = 14) exhibiting clinical signs of diarrhoea and from healthy calves were collected during 2007-2010 from subtropical (central India) and tarai area of western temperate Himalayan foothills (Uttarakhand). The dsRNA nature of the virus was confirmed by nuclease treatment (RNase A, RNaseT1 and DNase 1). PAGE results confirmed 3.67% (5/136) positivity for PBV, showing a typical genomic migration pattern with two discrete bands with size of approximately 2.4 and 1.7 kbps for the larger and smaller segments, respectively. Among the five PBV samples identified, three were from buffalo calves and one from cow calf exhibiting clinical signs of acute diarrhoea, while one sample from non-diarrhoeic buffalo calf also showed the presence of PBV. None of the samples showed dual infection of rotavirus and PBV. The preliminary findings indicate sporadic incidences of PBV in bovine calves and emphasize the need for the development of better diagnostics for early detection and genetic characterization of these emerging isolates of farm animals of economic significance.
File Format	HTM / HTML
ISSN	00494747
Issue Number	8
Volume Number	43
e-ISSN	15737438
Journal	Tropical Animal Health and Production
Language	English
Publisher	Springer
Publisher Date	2011-12-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Discipline Tropical Medicine Cattle Diseases Epidemiology Diarrhea Veterinary Feces Microbiology Picobirnavirus Isolation & Purification Rna Virus Infections Animals Buffaloes Cattle Virology Dairying Electrophoresis, Polyacrylamide Gel Incidence India Genetics Rna, Double-stranded Analysis Rna, Viral Rotavirus Rotavirus Infections Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Food Animals

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