NDLI: Effect of matrix elasticity on the maintenance of the chondrogenic phenotype.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Schuh, Elena Kramer, Jan Rohwedel, Jürgen Notbohm, Holger Müller, Ralph Gutsmann, Thomas Rotter, Nicole
Description	Country affiliation: Germany Author Affiliation: Schuh E ( Institute of Virology and Cell Biology, University of Lübeck , Lübeck, Germany .)
Abstract	The aim of this study was to examine the influence of matrix elasticity on the maintenance of the chondrogenic phenotype of chondrocytes cultured in monolayer. We used a two-dimensional culturing system in which polyacrylamide gels with different concentrations of bis-acrylamide were coated with collagen type I. Matrices with a Young's modulus of 4, 10, 40, and 100 kPa were produced, as determined by atomic force microscopy. Porcine chondrocytes were cultivated on these matrices at a low density for 7 days. The proliferation of cells was analyzed by 5-Bromo-2'-deoxy-uridine incorporation. Maintenance of the chondrogenic phenotype was analyzed by measuring collagen type I, type II, and aggrecan gene expression, immunofluorescence staining for collagen type II, and phalloidin staining for actin filaments. Cellular proliferation and actin organization were decreased on matrices of 4 kPa compared with stiffer substrates. The differentiated phenotype of the chondrocytes grown on matrices of 4 kPa was stabilized, indicated by higher collagen type II and aggrecan, and lower collagen type I expression. These findings indicate that chondrocytes sense the elasticity of the matrix and might be used for the design of scaffolds with mechanical properties specifically tailored to support the chondrogenic phenotype in tissue engineering applications.
File Format	HTM / HTML
ISSN	21524947
Issue Number	4
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Chondrocytes Cytology Physiology Extracellular Matrix Tissue Engineering Methods Acrylic Resins Actins Metabolism Animals Base Sequence Cell Proliferation Cells, Cultured Chondrogenesis Genetics Collagen Collagen Type I Collagen Type Ii Dna Primers Elastic Modulus Elasticity Gels Phenotype Sus Scrofa Tissue Scaffolds Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article

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