NDLI: Controlled release of bone morphogenetic protein-2 enhances recruitment of osteogenic progenitor cells for de novo generation of bone tissue.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Kimura, Yu Miyazaki, Nobuhiko Hayashi, Naoki Otsuru, Satoru Tamai, Katsuto Kaneda, Yasufumi Tabata, Yasuhiko
Description	Country affiliation: Japan Author Affiliation: Kimura Y ( Department of Biomaterials, Institute for Frontier Medical Sciences, Kyoto University , Kyoto, Japan .)
Abstract	The objective of this study was to evaluate the cellular contribution to the phenomenon of de novo generation of bone tissue induced by the controlled release of bone morphogenetic protein-2 (BMP-2). Gelatin hydrogels (2 mg) incorporating BMP-2 (3 microg) with different water contents were subcutaneously implanted into the back of enhanced green fluorescent protein-chimeric mice to induce the ectopic de novo generation of bone tissue. The hydrogels incorporating BMP-2 could release BMP-2 at different time profiles. When evaluated radiologically and histologically, the ectopic de novo generation of bone tissue was induced by the controlled release of BMP-2 from the hydrogels around the hydrogel-implanted site. The relative percentage number of green fluorescent protein- to osteocalcin-positive cells recruited into the de novo generated bone tissue depended on the BMP-2 release profile. The higher the percentage, the stronger was the de novo generation of bone tissue. These findings indicate that bone marrow-derived osteoblast progenitor cells were recruited from the blood circulation by BMP-2 release and consequently contributed to the ectopic de novo generation of bone tissue. It is conceivable that the local concentration of BMP-2 modifies the recruitment profile of progenitor cells with an osteogenic potential around the release site of BMP-2, resulting in regulated volume of de novo generated bone tissue.
File Format	HTM / HTML
ISSN	21524947
Issue Number	4
Volume Number	16
e-ISSN	21524955
Journal	Tissue Engineering Part A
Language	English
Publisher	Mary Ann Liebert, Inc.
Publisher Date	2010-04-01
Publisher Place	United States
Access Restriction	Subscribed
Subject Keyword	Discipline Biotechnology Adult Stem Cells Cytology Drug Effects Bone Morphogenetic Protein 2 Administration & Dosage Osteoblasts Osteogenesis Tissue Engineering Methods Physiology Animals Genetics Bone Regeneration Cell Movement Delayed-action Preparations Drug Implants Gelatin Green Fluorescent Proteins Humans Hydrogels Male Mice Mice, Inbred C57bl Mice, Transgenic Recombinant Proteins Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article

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