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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Bella, A. Sousa, N. M. Dehimi, M. L. Beckers, J. F. |
| Description | Country affiliation: Belgium Author Affiliation: Bella A ( Laboratory of Endocrinology and Animal Reproduction, Faculty of Veterinary Medicine, University of Liege, B-4000 Liege, Belgium.) |
| Abstract | The objective of this work was to investigate the expression of gastric aspartic proteinases in fundic and pyloric mucosa removed from bovine fetuses. For this purpose, fractions issued from classical biochemical protocols were analyzed by proteolytic method, by PAG-RIA and by Western blot with the use of antisera raised against both pepsinogens and PAG. A strong reaction of proteins extracted from the fundic mucosa collected at the beginning of pregnancy was revealed with both anti-bPAG-I and anti-bPAG-II antisera, suggesting the expression of pepsinogen F in bovine species. Concerning pyloric mucosa, the analysis by Western blot highlighted a very strong immunoreaction with the anti-bovine chymosin serum. Amino-terminal sequencing allowed to identify bovine fetuin and albumin in fundic extracts, chymosin in the pyloric mucosa extracts, as well as some unknown proteins in both mucosa. Despite no N-terminal microsequence corresponding to the hypothetical pepsinogen F could be identified, it cannot be excluded that an existing bovine pepsinogen F-like molecule could be degraded during the purification procedure or that co-purified proteins could be responsible for masking its N-terminal microsequence. |
| File Format | HTM / HTML |
| ISSN | 00345288 |
| Issue Number | 3 |
| Volume Number | 92 |
| e-ISSN | 15322661 |
| Journal | Research in Veterinary Science |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2012-06-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Veterinary Medicine Cattle Physiology Chymosin Metabolism Fetus Gastric Mucosa Glycoproteins Pepsinogen A Animals Genetics Female Embryology Gene Expression Regulation, Developmental Pregnancy Species Specificity Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Veterinary |
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