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Evaluation of a microsphere-based immunofluorescence assay for the determination of Immunoglobulin A concentrations in cerebrospinal fluid of dogs.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Roerig, A. Carlson, R. Tipold, A.
Description	Country affiliation: Germany Author Affiliation: Roerig A ( Department of Small Animal Medicine and Surgery, University of Veterinary Medicine Hannover, Hannover, Germany. andrea.roerig@tiho-hannover.de)
Abstract	The simultaneous increase of immunoglobulin A (IgA) in serum and cerebrospinal fluid (CSF) is a characteristic finding in dogs suffering from canine steroid-responsive meningitis-arteritis (SRMA). The study aimed at developing and evaluating a microsphere-based immunofluorescence assay (MIA) for the measurement of IgA, trying to fulfill the need of a quicker method using only small volumes of CSF. Microsphere beads were coated with goat-anti-dog IgA antibodies and bound IgA was detected by a mouse-anti-dog IgA antibody in combination with a PE-labeled goat-anti-mouse IgG. CSF from 44 dogs were tested for IgA and compared with an in-house utilized ELISA. Using clinical relevant reference ranges, the new method showed a good agreement (84.17%) with the ELISA. A method comparison revealed a moderate agreement only. These findings indicate that the MIA will not replace the ELISA, but it opens the possibility for further research with microsphere-based assays.
File Format	HTM / HTML
ISSN	00345288
Issue Number	1
Volume Number	94
e-ISSN	15322661
Journal	Research in Veterinary Science
Language	English
Publisher	Elsevier
Publisher Date	2013-02-01
Publisher Place	Great Britain (UK)
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Veterinary Medicine Dogs Cerebrospinal Fluid Fluorescent Antibody Technique Veterinary Immunoglobulin A Microspheres Animals Arteritis Immunology Dog Diseases Enzyme-linked Immunosorbent Assay Methods Meningitis Mice Journal Article
Content Type	Text
Resource Type	Article
Subject	Veterinary

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