NDLI: Protocol optimization for long-term liquid storage of goat semen in a chemically defined extender.

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Protocol optimization for long-term liquid storage of goat semen in a chemically defined extender.

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Protocol optimization for long-term liquid storage of goat semen in a chemically defined extender.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Zhao, B-T Han, D. Xu, C-L Luo, M-J Chang, Z-L Tan, J-H
Description	Country affiliation: China Author Affiliation: Zhao BT ( College of Animal Science and Veterinary Medicine, Shandong Agricultural University, Tai-an City, Shandong Province 271018, China.)
Abstract	A specific problem in the preservation of goat semen has been the detrimental effect of seminal plasma on the viability of spermatozoa in extenders containing egg yolk or milk. The use of chemically defined extenders will have obvious advantages in liquid storage of buck semen. Our previous study showed that the self-made mZAP extender performed better than commercial extenders, and maintained a sperm motility of 34% for 9 days and a fertilizing potential for successful pregnancies for 7 days. The aim of this study was to extend the viability and fertilizing potential of liquid-stored goat spermatozoa by optimizing procedures for semen processing and storage in the mZAP extender. Semen samples collected from five goat bucks of the Lubei White and Boer breeds were diluted with the extender, cooled and stored at 5 degrees C. Stored semen was evaluated for sperm viability parameters, every 48 h of storage. Data from three ejaculates of different bucks were analysed for each treatment. The percentage data were arcsine-transformed before being analysed with anova and Duncan's multiple comparison test. While cooling at the rate of 0.1-0.25 degrees C/min did not affect sperm viability parameters, doing so at the rate of 0.6 degrees C/min from 30 to 15 degrees C reduced goat sperm motility and membrane integrity. Sperm motility and membrane integrity were significantly higher in semen coated with the extender containing 20% egg yolk than in non-coated semen. Sperm motility, membrane integrity and acrosomal intactness were significantly higher when coated semen was 21-fold diluted than when it was 11- or 51-fold diluted and when extender was renewed at 48-h intervals than when it was not renewed during storage. When goat semen coated with the egg yolk-containing extender was 21-fold diluted, cooled at the rate of 0.07-0.25 degrees C/min, stored at 5 degrees C and the extender renewed every 48 h, a sperm motility of 48% was maintained for 13 days, and an in vitro-fertilizing potential similar to that of fresh semen was maintained for 11 days.
File Format	HTM / HTML
ISSN	09366768
Issue Number	6
Volume Number	44
e-ISSN	14390531
Journal	Reproduction in Domestic Animals
Language	English
Publisher	Wiley
Publisher Date	2009-12-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Reproductive Medicine Discipline Veterinary Medicine Discipline Zoology Goats Physiology Semen Preservation Veterinary Semen Animals Cell Membrane Drug Effects Fertilization In Vitro Male Methods Specimen Handling Sperm Motility Spermatozoa Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Animal Science and Zoology Endocrinology Biotechnology

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