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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sun, Run-Ze Pan, Qiu-Hong Duan, Chang-Qing Wang, Jun |
| Description | Country affiliation: China Author Affiliation: Sun RZ ( Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.); Pan QH ( Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.); Duan CQ ( Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China.); Wang J ( Center for Viticulture and Enology, College of Food Science and Nutritional Engineering, China Agricultural University, Beijing, 100083, China. Electronic address: jun_wang@cau.edu.cn.) |
| Abstract | Flavonoid 3'-hydroxylase (F3'H), a member of cytochrome P450 protein family, introduces B-ring hydroxyl group in the 3' position of the flavonoid. In this study, the cDNA sequence of a F3'H gene (VviF3'H), which contains an open reading frame of 1530 bp encoding a polypeptide of 509 amino acids, was cloned and characterized from Vitis vinifera L. cv. Cabernet Sauvignon. VviF3'H showed high homology to known F3'H genes, especially F3'Hs from the V. vinifera reference genome (Pinot Noir) and lotus. Expression profiling analysis using real-time PCR revealed that VviF3'H was ubiquitously expressed in all tested tissues including berries, leaves, flowers, roots, stems and tendrils, suggesting its important physiological role in plant growth and development. Moreover, the transcript level of VviF3'H gene in grape berries was relatively higher at early developmental stages and gradually decreased during véraison, and then increased in the mature phase. In addition, the promoter of VviF3'H was isolated by using TAIL-PCR. Yeast one-hybrid screening of the Cabernet Sauvignon cDNA library and subsequent in vivo/vitro validations revealed the interaction between VviF3'H promoter and several transcription factors, including members of HD-Zip, NAC, MYB and EIN families. A transcriptional regulation mechanism of VviF3'H expression is proposed for the first time. |
| File Format | HTM / HTML |
| ISSN | 09819428 |
| Volume Number | 97 |
| e-ISSN | 18732690 |
| Journal | Plant Physiology and Biochemistry |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2015-12-01 |
| Publisher Place | France |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Botany Discipline Biochemistry Cytochrome P-450 Enzyme System Metabolism Flavonoids Gene Expression Regulation, Enzymologic Plant Proteins Vitis Enzymology Amino Acid Sequence Base Sequence Genetics Flowers Radiation Effects Fruit Gene Expression Regulation, Plant Molecular Sequence Data Phylogeny Plant Leaves Plant Roots Plant Stems Promoter Regions, Genetic Sequence Alignment Sequence Analysis, Dna Transcription Factors Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Genetics Physiology Plant Science |
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