NDLI: MALDI sample preparation: the ultra thin layer method.

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MALDI sample preparation: the ultra thin layer method.

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Year: 2007 Issue: 2

MALDI sample preparation: the ultra thin layer method.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Fenyo, David Wang, Qingjun DeGrasse, Jeffrey A. Padovan, Julio C. Cadene, Martine Chait, Brian T.
Description	Country affiliation: United States Author Affiliation: Fenyo D ( Laboratory of Mass Spectrometry and Gaseous Ion Chemistry, Rockefeller University, USA. fenyo@rockefeller.edu)
Abstract	This video demonstrates the preparation of an ultra-thin matrix/analyte layer for analyzing peptides and proteins by Matrix-Assisted Laser Desorption Ionization Mass Spectrometry (MALDI-MS) (1, 2). The ultra-thin layer method involves the production of a substrate layer of matrix crystals (alpha-cyano-4-hydroxycinnamic acid) on the sample plate, which serves as a seeding ground for subsequent crystallization of a matrix/analyte mixture. Advantages of the ultra-thin layer method over other sample deposition approaches (e.g. dried droplet) are that it provides (i) greater tolerance to impurities such as salts and detergents, (ii) better resolution, and (iii) higher spatial uniformity. This method is especially useful for the accurate mass determination of proteins. The protocol was initially developed and optimized for the analysis of membrane proteins and used to successfully analyze ion channels, metabolite transporters, and receptors, containing between 2 and 12 transmembrane domains (2). Since the original publication, it has also shown to be equally useful for the analysis of soluble proteins. Indeed, we have used it for a large number of proteins having a wide range of properties, including those with molecular masses as high as 380 kDa (3). It is currently our method of choice for the molecular mass analysis of all proteins. The described procedure consistently produces high-quality spectra, and it is sensitive, robust, and easy to implement.
File Format	HTM / HTML
e-ISSN	1940087X
DOI	10.3791/192
Journal	Journal of Visualized Experiments
Issue Number	3
Language	English
Publisher	MyJove Corp.
Publisher Date	2007-01-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Physical Sciences Discipline Life Sciences Discipline Medicine Peptides Proteins Spectrometry, Mass, Matrix-assisted Laser Desorption-ionization Instrumentation Video-audio Media
Content Type	Text
Resource Type	Article

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