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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Burette, Alain C. Lesperance, Thomas Crum, John Martone, Maryann Volkmann, Niels Ellisman, Mark H. Weinberg, Richard J. |
| Description | Country affiliation: United States Author Affiliation: Burette AC ( Department of Cell & Developmental Biology, University of North Carolina, Chapel Hill, North Carolina 27599, USA. alain.burette@gmail.com) |
| Abstract | Synaptic function depends on interactions among sets of proteins that assemble into complex supramolecular machines. Molecular biology, electrophysiology, and live-cell imaging studies have provided tantalizing glimpses into the inner workings of the synapse, but fundamental questions remain regarding the functional organization of these 'nano-machines.' Electron tomography reveals the internal structure of synapses in three dimensions with exceptional spatial resolution. Here we report results from an electron tomographic study of axospinous synapses in neocortex and hippocampus of the adult rat, based on aldehyde-fixed material stabilized with tannic acid in lieu of postfixation with osmium tetroxide. Our results provide a new window into the structural basis of excitatory synaptic processing in the mammalian brain. |
| File Format | HTM / HTML |
| ISSN | 00219967 |
| e-ISSN | 10969861 |
| DOI | 10.1002/cne.23067 |
| Journal | Journal of Comparative Neurology |
| Issue Number | 12 |
| Volume Number | 520 |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2012-08-15 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Neurology Electron Microscope Tomography Synapses Ultrastructure Animals Artifacts Dendritic Spines Physiology Osmium Tetroxide Post-synaptic Density Presynaptic Terminals Rats, Sprague-dawley Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Neuroscience |
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