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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Sivagurunathan, Periyasamy Sen, Biswarup Lin, Chiu-Yue |
| Description | Country affiliation: Taiwan Author Affiliation: Sivagurunathan P ( Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan.); Sen B ( Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan); Lin CY ( Department of Environmental Engineering and Science, Feng Chia University, Taichung 40724, Taiwan) |
| Abstract | The effect of individual and combined mixed culture on dark fermentative hydrogen production performance was investigated. Mixed cultures from cow dung (C1), sewage sludge (C2), and pig slurry (C3) were enriched under strict anaerobic conditions at 37°C with glucose as the sole carbon source. Biochemical hydrogen production test in peptone-yeast-glucose (PYG) and basal medium was performed for individual mixed cultures (C1, C2 and C3) and their combinations (C1-C2, C2-C3, C1-C3 and C1-C2-C3) at a glucose concentration of 10 g/L, 37°C and initial pH 7. Maximum hydrogen yields (HY) of 2.0 and 1.86 [Formula: see text] by C2, and 1.98 and 1.95 mol(H2)/mol(glucose) by C2-C3 were obtained in PYG and basal medium, respectively. Butyrate and acetate were the major soluble metabolites produced by all the cultures, and the ratio of butyrate to acetate was â¼2 fold higher in basal medium than PYG medium, indicating strong influence of media formulation on glucose catabolism. The major hydrogen-producing bacterial strains, observed in all mixed cultures, belonged to Clostridium butyricum, C. saccharobutylicum, C. tertium and C. perfringens. The hydrogen production performance of the combined mixed culture (C2-C3) was further evaluated on beverage wastewater (10 g/L) at pH 7 and 37°C. The results showed an HY of 1.92 mol(H2)/mol(glucose-equivalent). Experimental evidence suggests that hydrogen fermentation by mixed culture combination could be a novel strategy to improve the HY from industrial wastewater. |
| File Format | HTM / HTML |
| ISSN | 13891723 |
| Issue Number | 2 |
| Volume Number | 117 |
| e-ISSN | 13474421 |
| Journal | Journal of Bioscience and Bioengineering |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-02-01 |
| Publisher Place | Japan |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Biomedical Engineering Discipline Microbiology Batch Cell Culture Techniques Bioreactors Microbiology Fermentation Hydrogen Metabolism Acetates Animals Beverages Butyrates Cattle Clostridium Classification Genetics Isolation & Purification Feces Female Food Industry Glucose Hydrogen-ion Concentration Industrial Waste Phylogeny Sewage Swine Waste Water Chemistry Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Bioengineering Applied Microbiology and Biotechnology Biotechnology |
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