NDLI: Combined effects of flow-induced shear stress and micropatterned surface morphology on neuronal differentiation of human mesenchymal stem cells.

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Combined effects of flow-induced shear stress and micropatterned surface morphology on neuronal differentiation of human mesenchymal stem cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Jeon, Kang Jin Park, So Hee Shin, Ji Won Kang, Yun Gyeong Hyun, Jin-Sook Oh, Min Jae Kim, Seon Yeon Shin, Jung-Woog
Description	Author Affiliation: Jeon KJ ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Park SH ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Shin JW ( Cardiovascular and Metabolic Disease Center, Inje University, Busan 633-165, Republic of Korea.); Kang YG ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Hyun JS ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Oh MJ ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Kim SY ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea.); Shin JW ( Department of Biomedical Engineering, Inje University, Gimhae, Gyeongnam 621-749, Republic of Korea)
Abstract	This study investigated the combined effects of surface morphology and flow-induced shear stress on the neuronal differentiation of human mesenchymal stem cells. First, to examine the effect of surface morphology, three patterns were fabricated using photolithography and compared to the flat substrate. After selecting the most effective surface pattern, flow-induced shear stresses (0.10 and 0.25 Pa) were engaged parallel to the direction of the grooves. The degrees of alignment and neurite outgrowth were measured using digital image processing techniques for up to 10 days. Functional evaluations were also performed by monitoring the intracellular calcium concentration and the expression of synaptophysin, ß-tubulin III, and MAP2. Based on these analyses, the pattern of 5 µm/5 µm/3 µm for groove/ridge/depth, respectively, was selected. Next, shear stresses (0.00, 0.10, 0.25 Pa) were applied to the cells on the selected substrate. The shear stresses affected the expression of those markers. The outgrowth measurements indicated that the shear stresses were effective at day 7. However, the effect of shear stresses tended to decrease at day 10. More cells showed higher calcium concentrations under 0.10 Pa. The alignment was also confirmed. Taken together, these results indicated that a shear stress of 0.10 Pa on the substrate of 5 µm was most effective. Therefore, such combination of mechanical stimuli and surface pattern is expected to promote neuronal differentiation with regard to functional and morphological changes.
File Format	HTM / HTML
ISSN	13891723
Issue Number	2
Volume Number	117
e-ISSN	13474421
Journal	Journal of Bioscience and Bioengineering
Language	English
Publisher	Elsevier
Publisher Date	2014-02-01
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Discipline Microbiology Cell Differentiation Mesenchymal Stromal Cells Cytology Neural Stem Cells Neurons Stress, Mechanical Biological Markers Analysis Calcium Metabolism Cell Shape Humans Rheology Surface Properties Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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