NDLI: Characterization of an alkalophilic extracellular chitosanase from Bacillus cereus GU-02.

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Characterization of an alkalophilic extracellular chitosanase from Bacillus cereus GU-02.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Goo, Bon Geun Park, Jae Kweon
Description	Author Affiliation: Goo BG ( Department of Pharmaceutical Science, Gachon University, 191 Hambakmoeiro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea.); Park JK ( Department of Life Science, College of Bionano, Gachon University, 191 Hambakmoeiro, Yeonsu-dong, Yeonsu-gu, InCheon 406-799, Republic of Korea. Electronic address: jkpark@gachon.ac.kr.)
Abstract	An alkalophilic extracellular chitosanase (ACTase) was characterized from the culture supernatant of Bacillus cereus GU-02. Kinetic properties of ACTase produced from B. cereus GU-02 after cultivation in anaerobic condition, alkaline medium (pH 10) at 37°C for 3 days were investigated. ACTase was found to be stable in alkaline pH range from 8 to 10. Interestingly, optimum pH and temperature were estimated to be 10 and 37°C, respectively, where ACTase showed chitosan degrading activity (87%), which was enhanced by 15% in the presence of calcium ions (8 mM). The ACTase produced from B. cereus GU-02 was partially purified from the culture supernatant, and its enzymatic activity was kinetically characterized. The Vmax and Km were estimated with a chitosan (degree of deacetylation, DD 92% as substrate) as 0.038 U/min/µg protein and 0.327 µM, respectively. A combination of the TLC and MALDI-TOF MS results showed that the chitosan oligosaccharides obtained from the hydrolysis of high molecular weight chitosan (HMWC) by ACTase of the B. cereus GU-2 comprise oligomers with degree of polymerization (DP) mainly from dimers to pentamers. High production of ACTase and chitooligosaccharides may be useful for various industrial and biological applications.
File Format	HTM / HTML
ISSN	13891723
Issue Number	6
Volume Number	117
e-ISSN	13474421
Journal	Journal of Bioscience and Bioengineering
Language	English
Publisher	Elsevier
Publisher Date	2014-06-01
Publisher Place	Japan
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biomedical Engineering Discipline Microbiology Bacillus Cereus Enzymology Bacterial Proteins Chemistry Glycoside Hydrolases Biosynthesis Isolation & Purification Carbohydrate Conformation Chitosan Hydrogen-ion Concentration Hydrolysis Kinetics Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Bioengineering Applied Microbiology and Biotechnology Biotechnology

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