NDLI: Real-time polymerase chain reaction for detection of Isospora belli in stool samples.

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Real-time polymerase chain reaction for detection of Isospora belli in stool samples.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Ten Hove, Robert-jan Van Lieshout, Lisette Brienen, Eric A. T. Perez, M. Arantza Verweij, Jaco J.
Description	Country affiliation: Netherlands Author Affiliation: ten Hove RJ ( Department of Parasitology, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands. r.ten_hove@lumc.nl)
Abstract	A real-time polymerase chain reaction (PCR) assay targeting the internal transcribed spacer 2 region of the ribosomal RNA gene was developed for the detection of Isospora belli DNA in fecal samples, including an internal control to detect inhibition during the amplification process. The assay was performed on species-specific DNA controls (n = 27) and a range of positive (n = 21) and negative (n = 120) stool samples, and achieved 100% specificity and sensitivity. The simple fecal sample collection procedure, the high-throughput potential, and the possibility of quantification makes the I. belli real-time PCR assay a powerful diagnostic tool for epidemiologic studies with possibilities for extension to other helminthes and protozoa using additional molecular targets. In addition, this Isospora PCR could augment the clinical laboratory diagnosis of isosporiasis, in particular, in patients with a travel history to developing countries.
File Format	HTM / HTML
ISSN	07328893
Issue Number	3
Volume Number	61
e-ISSN	18790070
Journal	Diagnostic Microbiology and Infectious Disease
Language	English
Publisher	Elsevier
Publisher Date	2008-07-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Feces Parasitology Isospora Isolation & Purification Isosporiasis Diagnosis Polymerase Chain Reaction Methods Animals Dna, Protozoan Genetics Dna, Ribosomal Spacer Humans Standards Reference Standards Sensitivity And Specificity Evaluation Studies Journal Article
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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