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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Mugasa, Claire M. Katiti, Diana Boobo, Alex Lubega, George W. Schallig, Henk D. F. H. Matovu, Enock |
| Description | Country affiliation: Uganda Author Affiliation: Mugasa CM ( School of Biosecurity Biotechnical Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University Kampala, Uganda.); Katiti D ( School of Biosecurity Biotechnical Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University Kampala, Uganda.); Boobo A ( School of Biosecurity Biotechnical Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University Kampala, Uganda.); Lubega GW ( School of Biosecurity Biotechnical Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University Kampala, Uganda.); Schallig HD ( Koninklijk Instituut voor de Tropen (KIT)/Royal Tropical Institute, KIT Biomedical Research, Department of Parasitology, Amsterdam, The Netherlands.); Matovu E ( School of Biosecurity Biotechnical Laboratory Sciences, College of Veterinary Medicine, Animal Resources and Biosecurity (COVAB), Makerere University Kampala, Uganda. Electronic address: matovue@vetmed.mak.ac.ug.) |
| Abstract | Diagnosis of human African trypanosomiasis (HAT) using molecular tests should ideally achieve high sensitivity without compromising specificity. This study compared 2 simplified tests, nucleic acid sequence-based amplification (NASBA) combined with oligochromatography (OC) and loop-mediated isothermal amplification (LAMP), executed on 181 blood samples from 65 Trypanosoma brucei gambiense HAT patients, 86 controls, and 30 serological suspects from Uganda. Basing on the composite reference standard, the diagnostic sensitivity and specificity of NASBA were 93.9% (95% confidence interval [CI] = 84.9-98.3%) and 100% (95% CI = 94.9-100%), respectively. The same parameters for LAMP were 76.9% (95% CI = 64.8-86.5%) and 100% (95% CI = 91.6-100%), respectively. The level of agreement between LAMP and microscopy was good with a kappa (κ) value of 79.2% (95% CI = 69.4-88.9%), while that of NASBA-OC/microscopy was very good (κ value 94.6%; 95% CI = 89.3-99.8%). The sensitivity of NASBA-OC was significantly higher than that of LAMP (Z = 2.723; P = 0.007). These tests have potential application to HAT surveillance. |
| File Format | HTM / HTML |
| ISSN | 07328893 |
| Issue Number | 2 |
| Volume Number | 78 |
| e-ISSN | 18790070 |
| Journal | Diagnostic Microbiology and Infectious Disease |
| Language | English |
| Publisher | Elsevier |
| Publisher Date | 2014-02-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Microbiology Discipline Communicable Diseases Nucleic Acid Amplification Techniques Methods Trypanosoma Classification Genetics Trypanosomiasis, African Diagnosis Case-control Studies Humans Reproducibility Of Results Sensitivity And Specificity Trypanosoma Brucei Gambiense Comparative Study Journal Article Research Support, Non-u.s. Gov't |
| Content Type | Text |
| Resource Type | Article |
| Subject | Infectious Diseases Microbiology (medical) |
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