NDLI: Use of quantitative 16S rRNA PCR to determine bacterial load does not augment conventional cerebrospinal fluid (CSF) cultures among children undergoing treatment for CSF shunt infection.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Simon, Tamara D. Van Yserloo, Brian Nelson, Kevin Gillespie, David Jensen, Randy McAllister, James P. Riva-Cambrin, Jay Stockmann, Chris Daly, Judy A. Blaschke, Anne J.
Description	Author Affiliation: Simon TD ( Department of Pediatrics, Division of Hospital Medicine, University of Washington/Seattle Children's Hospital, Seattle, WA, USA. Electronic address: Tamara.Simon@seattlechildrens.org.); Van Yserloo B ( Virus, Molecular Biology and Cell Core, Diabetes and Endocrinology Research Center, University of Washington, Seattle, WA, USA.); Nelson K ( Department of Pediatrics, University of Utah, Salt Lake City, UT, USA.); Gillespie D ( Department of Neurosurgery, University of Utah, Salt Lake City, UT, USA.); Jensen R ( Department of Neurosurgery, University of Utah, Salt Lake City, UT, USA.); McAllister JP ( Department of Neurosurgery, University of Utah, Salt Lake City, UT, USA.); Riva-Cambrin J ( Department of Neurosurgery, University of Utah, Salt Lake City, UT, USA.); Stockmann C ( Department of Pediatrics, University of Utah, Salt Lake City, UT, USA.); Daly JA ( Microbiology Laboratory, Primary Children's Medical Center, Salt Lake City, UT, USA); Blaschke AJ ( Department of Pediatrics, University of Utah, Salt Lake City, UT, USA.)
Abstract	The aim of this study was to develop a quantitative 16S rRNA assay for determination of bacterial nucleic acid load in cerebrospinal fluid (CSF) shunt infection and to compare quantitative 16S rRNA polymerase chain reaction (PCR) findings to those of conventional bacterial culture in patients treated for CSF shunt infection. We developed a quantitative 16S rRNA PCR assay that detected bacterial load across a range of 2.5 × 10(9) down to 2.5 × 10(4) 16S copies/mL CSF under experimental conditions for numerous Gram-positive and Gram-negative organisms. However, when applied to archived CSF samples from 25 shunt infection episodes, correlations between positive bacterial culture and 16S rRNA levels were seen in only half of infections, and 16S rRNA levels dropped precipitously after an initial peak on the first day of sample collection. Bacterial load measured using 16S rRNA PCR does not provide sufficient information beyond bacterial culture to inform CSF shunt infection treatment.
File Format	HTM / HTML
ISSN	07328893
e-ISSN	18790070
DOI	10.1016/j.diagmicrobio.2013.06.027
Journal	Diagnostic Microbiology and Infectious Disease
Issue Number	2
Volume Number	78
Language	English
Publisher	Elsevier
Publisher Date	2014-02-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Microbiology Discipline Communicable Diseases Bacteria Genetics Bacterial Infections Diagnosis Microbiology Bacterial Load Cerebrospinal Fluid Shunts Adverse Effects Cerebrospinal Fluid Rna, Ribosomal, 16s Adolescent Anti-bacterial Agents Therapeutic Use Classification Drug Therapy Real-time Polymerase Chain Reaction
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Microbiology (medical)

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