NDLI: Determination of gross chromosomal rearrangement rates.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Putnam, Christopher D. Kolodner, Richard D.
Description	Country affiliation: United States Author Affiliation: Putnam CD ( Ludwig Institute for Cancer Research, La Jolla, CA 92093-2385, USA. cdputnam@ucsd.edu)
Abstract	Cells devote a significant amount of metabolism to maintaining the stability of their genome and to preventing inappropriate chromosomal rearrangements that are characteristic of many cancers. A simple genetic assay using haploid derivatives of the yeast Saccharomyces cerevisiae provides a means to quantitatively measure the rate at which gross chromosomal rearrangements (GCRs) accumulate in different genetic backgrounds. This assay measures the rate of simultaneous inactivation of CAN1 and URA3 markers placed on a nonessential end of a yeast chromosome and in principle can be implemented in any haploid strain. Rearrangements detected with this assay include broken chromosomes healed by de novo telomere additions and a spectrum of inter- and intrachromosomal fusion events. The GCR assay allows for detailed analysis of the contributions of individual genes and different pathways in the suppression of genomic instability.
File Format	HTM / HTML
ISSN	19403402
e-ISSN	15596095
DOI	10.1101/pdb.prot5492
Journal	Cold Spring Harbor Protocols
Issue Number	9
Volume Number	2010
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2010-09-01
Publisher Place	United States
Access Restriction	Open
Subject Keyword	Discipline Clinical Laboratory Techniques Chromosomes, Fungal Dna, Fungal Genetics Genetic Techniques Genomic Instability Saccharomyces Cerevisiae Amino Acid Transport Systems, Basic Chromosomal Instability Gene Rearrangement Gene Silencing Cytology Saccharomyces Cerevisiae Proteins
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Molecular characterization of a chromosomal rearrangement involved in the adaptive evolution of yeast strains.

Mutator genes for suppression of gross chromosomal rearrangements identified by a genome-wide screening in Saccharomyces cerevisiae.

The 3'-->5' exonuclease of DNA polymerase delta can substitute for the 5' flap endonuclease Rad27/Fen1 in processing Okazaki fragments and preventing genome instability.

Suppression of gross chromosomal rearrangements by yKu70-yKu80 heterodimer through DNA damage checkpoints.

Topoisomerase 1 provokes the formation of short deletions in repeated sequences upon high transcription in Saccharomyces cerevisiae.

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Role of the GATA factors Gln3p and Nil1p of Saccharomyces cerevisiae in the expression of nitrogen-regulated genes.

Mph1p promotes gross chromosomal rearrangement through partial inhibition of homologous recombination

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Determination of gross chromosomal rearrangement rates.

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