NDLI: The Use of Aequorin and Its Variants for $Ca^{2+}$ Measurements

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The Use of Aequorin and Its Variants for $Ca^{2+}$ Measurements

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Using Targeted Variants of Aequorin to Measure $Ca^{2+}$ Levels in Intracellular Organelles

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The Use of Aequorin and Its Variants for $Ca^{2+}$ Measurements

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Granatiero, Veronica Patron, Maria Tosatto, Anna Merli, Giulia Rizzuto, Rosario
Description	Country affiliation: Italy Author Affiliation: Granatiero V ( Department of Biomedical Sciences, University of Padua and CNR Neuroscience Institute, 35131 Padua, Italy.)
Abstract	$Ca^{2+}-sensitive$ photoproteins are ideal agents for measuring the $Ca^{2+}$ concentration $([Ca^{2+}])$ in intracellular organelles because they can be modified to include specific targeting sequences. Aequorin was the first $Ca^{2+}-sensitive$ photoprotein probe used to measure the $[Ca^{2+}]$ inside specific intracellular organelles in intact cells. Aequorin is a 22-kDa protein produced by the jellyfish Aequorea victoria. On the binding of $Ca^{2+}$ to three high-affinity sites in aequorin, an irreversible reaction occurs in which the prosthetic group is released and a photon is emitted. Aequorin has become widely used for intracellular $Ca^{2+}$ measurements because it offers many advantages: For example, it can be targeted with precision, functions over a wide range of $[Ca^{2+}],$ and shows low buffering capacity. In this article we describe the main characteristics of the aequorin probe and review the reasons why it is widely used to measure intracellular $[Ca^{2+}].$
File Format	HTM / HTML
ISSN	19403402
Issue Number	1
Volume Number	2014
e-ISSN	15596095
Journal	Cold Spring Harbor Protocols
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2014-01-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Aequorin Calcium Analysis Isolation & Purification Animals Cytosol Chemistry Hydrozoa Organelles Photons Protein Binding Introductory Journal Article Research Support, N.i.h., Extramural Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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Using Targeted Variants of Aequorin to Measure $Ca^{2+}$ Levels in Intracellular Organelles

Properties of genetically-encoded indicators used for cytosolic and organellar $Ca^{2+}$ measurements

Protocol 1: Verifying the function and localization of genetically-encoded $Ca^{2+}$ sensors and converting FRET ratios to $Ca^{2+}$ concentrations

GAP, an aequorin-based fluorescent indicator for imaging $Ca^{2+}$ in organelles

Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis

SAINT: probabilistic scoring of affinity purification-mass spectrometry data.

Semisynthetic photoprotein reporters for tracking fast $Ca^{2+}$ transients

$Ca^{2+}-Sensitive$ Fluorescent Dyes and Intracellular $Ca^{2+}$ Imaging

Synaptotagmin C2B Domain Regulates $Ca^{2+}-triggered$ Fusion in Vitro

The Use of Aequorin and Its Variants for $Ca^{2+}$ Measurements

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Using Targeted Variants of Aequorin to Measure $Ca^{2+}$ Levels in Intracellular Organelles

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Protocol 1: Verifying the function and localization of genetically-encoded $Ca^{2+}$ sensors and converting FRET ratios to $Ca^{2+}$ concentrations

GAP, an aequorin-based fluorescent indicator for imaging $Ca^{2+}$ in organelles

Isolation of rare recombinants without using selectable markers for one-step seamless BAC mutagenesis

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The Use of Aequorin and Its Variants for $Ca^{2+}$ Measurements