NDLI: Flash Photolysis of Caged $IP_{3}$ to Trigger Intercellular $Ca^{2+}$ Waves

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Electroporation Loading of Membrane-Impermeable Molecules to Investigate Intra- and Intercellular $Ca^{2+}$ Signaling

Flash Photolysis of Caged $IP_{3}$ to Trigger Intercellular $Ca^{2+}$ Waves

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Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular $Ca^{2+}$ Signaling

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Flash Photolysis of Caged $IP_{3}$ to Trigger Intercellular $Ca^{2+}$ Waves

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Decrock, Elke De Bock, Marijke Wang, Nan Bol, Mélissa Gadicherla, Ashish K. Leybaert, Luc
Description	Country affiliation: Belgium Author Affiliation: Decrock E ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.); De Bock M ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.); Wang N ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.); Bol M ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.); Gadicherla AK ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.); Leybaert L ( Department of Basic Medical Sciences, Physiology Group, Ghent University, 9000 Ghent, Belgium.)
Abstract	Caged $IP_{3}$ is an inactive form of the second messenger $IP_{3},$ consisting of the biologically active molecule linked to a cage group through a photolabile bond. This bond is cleaved by exposure to brief “flashes” of ultraviolet (UV) light, thereby releasing the active $IP_{3}$ molecule. The protection of caged $IP_{3}$ against metabolic transformation in combination with a defined time point of fast photoliberation of $IP_{3}$ provides an efficient way to temporally and spatially control the cytosolic release of $IP_{3}$ and subsequent increase of cytoplasmic $Ca^{2+}.$ These properties make it an ideal method for kinetic studies and also a well-suited procedure to initiate intercellular $Ca^{2+}$ waves from a point source of $IP_{3}.$ This protocol describes cell loading with membrane impermeable caged $IP_{3}$ and the UV flash illumination procedure.
File Format	HTM / HTML
ISSN	19403402
Issue Number	3
Volume Number	2015
e-ISSN	15596095
Journal	Cold Spring Harbor Protocols
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2015-03-02
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Inositol 1,4,5-trisphosphate Analogs & Derivatives Photolysis Ultraviolet Rays Animals Calcium Metabolism Cell Line Cytosol Chemistry Humans Radiation Effects Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
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Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular $Ca^{2+}$ Signaling

High-Throughput Analyses of $IP_{3}$ Receptor Behavior

Monitoring of $IP_{3}$ dynamics during the mechanical stimulation-induced intra- and intercellular $Ca^{2+}$ waves in HSY human parotid cell line

High-Throughput Functional Assays of $IP_{3}-Evoked$ $Ca^{2+}$ Release

Interactions of antagonists with subtypes of inositol 1,4,5-trisphosphate $(IP_{3})$ receptor

$IP_{3},$ a small molecule with a powerful message☆

Studying isoform-specific inositol 1,4,5-trisphosphate receptor function and regulation

The Clustering of Inositol 1,4,5-Trisphosphate $(IP_{3})$ Receptors Is Triggered by $IP_{3}$ Binding and Facilitated by Depletion of the $Ca^{2+}$ Store

Flash photolysis of caged inositol 1,4,5-trisphosphate activates plasma membrane calcium current in human T cells.

Flash Photolysis of Caged $IP_{3}$ to Trigger Intercellular $Ca^{2+}$ Waves

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Electroporation Loading and Flash Photolysis to Investigate Intra- and Intercellular $Ca^{2+}$ Signaling

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Flash photolysis of caged inositol 1,4,5-trisphosphate activates plasma membrane calcium current in human T cells.

Flash Photolysis of Caged $IP_{3}$ to Trigger Intercellular $Ca^{2+}$ Waves