NDLI: High-Throughput Analyses of $IP_{3}$ Receptor Behavior

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High-Throughput Analyses of $IP_{3}$ Receptor Behavior

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High-Throughput Analyses of $IP_{3}$ Receptor Behavior

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Taylor, Colin W. Tovey, Stephen C. Rossi, Ana M.
Description	Country affiliation: United kingdom Author Affiliation: Taylor CW ( Department of Pharmacology, University of Cambridge, Cambridge CB2 1PD, United Kingdom.)
Abstract	Inositol 1,4,5-trisphosphate receptors $(IP_{3}Rs)$ are intracellular $Ca^{2+}$ channels. They are expressed in most animal cells and mediate release of $Ca^{2+}$ from the endoplasmic reticulum (ER) in response to the many stimuli that evoke formation of inositol 1,4,5-trisphosphate $(IP_{3}).$ The opening of individual $IP_{3}Rs$ causes small, transient, local increases in cytosolic $Ca^{2+}$ concentration, and these events are the fundamental units of $Ca^{2+}$ signaling. These openings allow $Ca^{2+}$ signals to be selectively delivered by individual channels to the specific $Ca^{2+}$ sensors that evoke cellular responses. Stimulation of $IP_{3}Rs$ by the $Ca^{2+}$ they release allows these tiny events to grow into much larger ones by recruitment of neighboring $IP_{3}Rs.$ Understanding how $Ca^{2+}$ effectively and specifically regulates so many cellular processes demands an understanding of the interplay between $IP_{3}$ and $Ca^{2+}$ in controlling $IP_{3}R$ gating. Here, we briefly set the scene before introducing high-throughput methods that seek to address this issue.
File Format	HTM / HTML
ISSN	19403402
Issue Number	10
Journal	Cold Spring Harbor Protocols
Volume Number	2013
e-ISSN	15596095
Language	English
Publisher	Cold Spring Harbor Laboratory Press
Publisher Date	2013-10-01
Publisher Place	United States
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Clinical Laboratory Techniques Calcium Channels Metabolism Calcium Inositol 1,4,5-trisphosphate Receptors Inositol 1,4,5-trisphosphate Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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