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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Rivera, Maria C. Maguire, Bruce Lake, James A. |
| Description | Author Affiliation: Rivera MC ( Department of Biology and Center for the Study of Biological Complexity, Virginia Commonwealth University, Richmond, Virginia 23284); Maguire B ( Primary Pharmacology Group, Pfizer Global Research and Development, Groton, Connecticut 06340); Lake JA ( Department of Molecular, Cell and Developmental Biology and Department of Human Genetics, University of California, Los Angeles, California 90095.) |
| Abstract | Structural and functional studies of ribosomal subunits require the dissociation of intact ribosomes into individual small and large ribosomal subunits. The dissociation of the prokaryotic 70S ribosomes into the 50S and 30S subunits is achieved by dialysis against a buffer containing a lower $Mg^{2+}$ concentration. Eukaryotic 80S ribosomes are dissociated into 60S and 40S subunits by incubation in a buffer containing puromycin and higher KCl and $Mg^{2+}$ concentrations. |
| File Format | HTM / HTML |
| ISSN | 19403402 |
| Issue Number | 4 |
| Journal | Cold Spring Harbor Protocols |
| Volume Number | 2015 |
| e-ISSN | 15596095 |
| Language | English |
| Publisher | Cold Spring Harbor Laboratory Press |
| Publisher Date | 2015-04-01 |
| Publisher Place | United States |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Clinical Laboratory Techniques Centrifugation, Density Gradient Methods Ribosome Subunits Metabolism Animals Chloroplasts Sucrose Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry, Genetics and Molecular Biology |
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