NDLI: Structural elucidation of possible lutein oxidation products mediated through peroxyl radical inducer 2,2'-Azobis (2-methylpropionamidine) dihydrochloride: antioxidant and cytotoxic influence of oxidized lutein in HeLa cells.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Lakshminarayana, Rangaswamy Aruna, Gorusupudi Sathisha, Upparahalli V. Dharmesh, Shylaja M. Baskaran, Vallikannan
Description	Country affiliation: India Author Affiliation: Lakshminarayana R ( Department of Biochemistry and Nutrition, Central Food Technological Research Institute (CSIR), Mysore 570 020, India.)
Abstract	Aim of this study was to elucidate lutein oxidation products mediated through peroxyl radical inducer 2,2'-Azobis (2-methylpropionamidine) dihydrochloride (AAPH) and to study antioxidant and cytotoxic effects of oxidized lutein using liposome and HeLa cells. Lutein (20 µmol) with AAPH (5 mM) in liposome's was incubated at 37 °C in dark for 3 h, oxidized lutein products were characterized by LC-MS (APCI(+)) and studied for their free radical scavenging activity and cytotoxic effects in terms of cell viability, cellular glutathione, and malondialdehyde levels. AAPH mediated lutein fragmented ions were identified as 551 (M(+)+H(+)-H(2)O), 391 (M(+)+H(+)+O(2)-C(22)H(32)O) and 276 (M(+)+H(+)+O(2)-C(12)H(20)O) and its isomers as 13-Z lutein, 13-Z zeaxanthin, 13'-Z zeaxanthin and all-E zeaxanthin. Free radical scavenging activity of oxidized lutein was higher by 32.7% (IC(50), 2.64 µg) than lutein (IC(50), 5.28 µg). Oxidized lutein lowered the lipid peroxidation (21%), HeLa cells viability (22%) and glutathione levels (32%) than lutein. To conclude, the oxidized lutein may be highly reactive, since oxidation by AAPH results in peroxyl radical ions, which can react with conjugated polyene chain of lutein that could lead to higher antioxidant and cytotoxic effects on HeLa cells.
File Format	HTM / HTML
ISSN	00092797
Issue Number	2
Volume Number	203
e-ISSN	18727786
Journal	Chemico-Biological Interactions
Language	English
Publisher	Elsevier
Publisher Date	2013-04-25
Publisher Place	Ireland
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Discipline Biochemistry Discipline Pharmacology Amidines Pharmacology Lutein Chemistry Peroxides Metabolism Antineoplastic Agents Biphenyl Compounds Drug Interactions Free Radical Scavengers Hela Cells Humans Oxidation-reduction Picrates Journal Article Research Support, Non-u.s. Gov't
Content Type	Text
Resource Type	Article
Subject	Medicine Toxicology

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