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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Patel, Daxesh P. Sharma, Primal Sanyal, Mallika Singhal, Puran Shrivastav, Pranav S. |
| Description | Country affiliation: India Author Affiliation: Patel DP ( Department of Chemistry, School of Sciences, Gujarat University, Navrangpura, Ahmedabad 380009, Gujarat, India.) |
| Abstract | An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method has been developed for the determination of nifedipine in human plasma using nifedipine-d6 as the internal standard (IS). The plasma samples were prepared by solid-phase extraction on Phenomenex Strata-X cartridges employing 200 µL human plasma. Chromatography was carried out on Waters Acquity UPLC BEH C18 (50 × 2.1 mm, 1.7 µm particle size) analytical column under isocratic conditions using a mobile phase consisting of 4.0 mm ammonium acetate-acetonitrile (15:85, v/v). The precursor â product ion transitions for nifedipine (m/z 347.2 â 315.2) and IS (m/z 353.1 â 318.1) were monitored on a triple quadrupole mass spectrometer, operating in the multiple reaction monitoring and positive-ion mode. The method was validated over a wide dynamic concentration range of 0.050-150 ng/mL. Matrix effect was assessed by post-column analyte infusion and the mean extraction recovery was 95.6% across four quality control levels. The method is rugged and rapid with a total run time of 1.2 min and was applied to a bioequivalence study of 20 mg nifedipine tablet formulation in 30 healthy Indian subjects under fasting condition. Assay reproducibility was confirmed by reanalysis of 116 incurred samples. |
| File Format | HTM / HTML |
| ISSN | 02693879 |
| Issue Number | 12 |
| Volume Number | 26 |
| e-ISSN | 10990801 |
| Journal | Biomedical Chromatography |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2012-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Discipline Molecular Biology Discipline Biochemistry Discipline Pharmacology Chromatography, High Pressure Liquid Methods Nifedipine Blood Pharmacokinetics Tandem Mass Spectrometry Adult Area Under Curve Drug Stability High-throughput Screening Assays Humans Male Reproducibility Of Results Sensitivity And Specificity Solid Phase Extraction Therapeutic Equivalency Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Drug Discovery Clinical Biochemistry Biochemistry Molecular Biology Pharmacology |
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