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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Nirogi, Ramakrishna Kandikere, Vishwottam Komarneni, Prashanth Aleti, Raghupathi Padala, Nagasuryaprakash Kalaikadhiban, Ilayaraja Bhyrapuneni, Gopinadh Muddana, Nageswararao |
| Description | Country affiliation: India Author Affiliation: Nirogi R ( Pharmacokinetics and Drug Metabolism, Discovery Research, Suven Life Sciences Ltd, Serene Chambers, Road 5, Avenue 7, Banjara Hills, Hyderabad 500034, India. ramakrishna_nirogi@yahoo.co.in) |
| Abstract | A high-performance liquid chromatography/positive ion electrospray tandem mass spectrometry method for the simultaneous quantification of lamivudine, stavudine and nevirapine was developed and validated in dried blood spot (DBS) cards. The analytes were separated using an isocratic mobile phase on a reverse phase column and analyzed by MS/MS in the MRM mode using the respective [M + H]⺠ions, m/z 230-112 for lamivudine, m/z 225-127 for stavudine, m/z 267-226 for nevirapine, m/z 383-337 for zidovudine (IS). The lower limit of quantification was 1 ng/mL for both lamivudine and stavudine and 10 ng/mL for nevirapine. Acceptable precision and accuracy were obtained for concentrations over the standard curve range. The method was successfully applied to quantify them in a rat pharmacokinetic study in whole blood, plasma and DBS cards after a single oral co-administration at the dose of 10, 2 and 13 mg/kg for lamivudine, stavudine and nevirapine, respectively, to male Wistar rats. Following oral administration the pharmacokinetic results in all the matrices are in close agreement. Thus accomplishment of this method would facilitate the ease of collection of clinical samples on DBS cards for lamivudine, stavudine and nevirapine during human clinical trials and therapeutic drug monitoring. |
| File Format | HTM / HTML |
| ISSN | 02693879 |
| Issue Number | 12 |
| Volume Number | 26 |
| e-ISSN | 10990801 |
| Journal | Biomedical Chromatography |
| Language | English |
| Publisher | Wiley |
| Publisher Date | 2012-12-01 |
| Publisher Place | Great Britain (UK) |
| Access Restriction | One Nation One Subscription (ONOS) |
| Subject Keyword | Discipline Analytical Chemistry Discipline Molecular Biology Discipline Biochemistry Discipline Pharmacology Anti-retroviral Agents Blood Dried Blood Spot Testing Methods Animals Chemistry Pharmacokinetics Chromatography, High Pressure Liquid Drug Stability Lamivudine Male Nevirapine Rats Rats, Wistar Reproducibility Of Results Sensitivity And Specificity Stavudine Tandem Mass Spectrometry Journal Article |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Drug Discovery Clinical Biochemistry Biochemistry Molecular Biology Pharmacology |
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