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| Content Provider | World Health Organization (WHO)-Global Index Medicus |
|---|---|
| Author | Liao, Haini Zhang, Jie Shestopal, Svetlana Szabo, Gabor Castle, Anna Castle, David |
| Description | Country affiliation: United States Author Affiliation: Liao H ( Dept. of Cell Biology and Cell and Developmental Biology Program , Univ. of Virginia, Charlottesville, VA 22908, USA.) |
| Abstract | Five secretory carrier membrane proteins (SCAMP-1, -2, -3, -4, and -5) have been characterized in mammalian cells. Previously, SCAMP-1 and -2 have been implicated to function in exocytosis. RNA inhibitor-mediated deficiency of one or both of these SCAMPs interferes with dense core vesicle (DCV) exocytosis in neuroendocrine PC12 cells as detected by amperometry. Knockdowns of these SCAMPs each decreased the number and frequency of depolarization-induced exocytotic events. SCAMP-2 but not SCAMP-1 depletion also delayed the onset of exocytosis. Both knockdowns, however, altered fusion pore dynamics, increasing rapid pore closure and decreasing pore dilation. In contrast, knockdowns of SCAMP-3 and -5 only interfered with the frequency of fusion pore opening and did not affect the dynamics of newly opened pores. None of the knockdowns noticeably affected upstream events, including the distribution of DCVs near the plasma membrane and calcium signaling kinetics, although norepinephrine uptake/storage was moderately decreased by deficiency of SCAMP-1 and -5. Thus, SCAMP-1 and -2 are most closely linked to the final events of exocytosis. Other SCAMPs collaborate in regulating fusion sites, but the roles of individual isoforms appear at least partially distinct. |
| File Format | HTM / HTML |
| ISSN | 03636143 |
| e-ISSN | 15221563 |
| Journal | American Journal of Physiology - Cell Physiology |
| Issue Number | 3 |
| Volume Number | 294 |
| Language | English |
| Publisher | American Physiological Society |
| Publisher Date | 2008-03-01 |
| Publisher Place | United States |
| Access Restriction | Open |
| Subject Keyword | Discipline Cell Biology Carrier Proteins Metabolism Cell Membrane Exocytosis Membrane Proteins Neurons Secretory Vesicles Animals Calcium Signaling Genetics Kinetics Membrane Fusion Membrane Potentials Models, Neurological Norepinephrine Pc12 Cells Protein Isoforms Rna Interference Rna, Small Interfering Transfection Tritium Research Support, N.i.h., Extramural |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Physiology |
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