NDLI: Assessment of fungal diversity in deep-sea sediments by multiple primer approach.

Please wait, while we are loading the content...

A modified visual loop-mediated isothermal amplification method for diagnosis and differentiation of main pathogens from Mycobacterium tuberculosis complex.

Specific identification of pathogenic Yersinia enterocolitica by monoclonal antibodies generated against recombinant attachment invasion locus (rAil) protein.

Catalytic characteristics of plant-esterase from wheat flour

Selection of available suicide vectors for gene mutagenesis using chiA (a chitinase encoding gene) as a new reporter and primary functional analysis of chiA in Lysobacter enzymogenes strain OH11

Ethanol production from sweet sorghum juice in repeated-batch fermentation by Saccharomyces cerevisiae immobilized on corncob.

Exogenous isolation of conjugative plasmids from pesticide contaminated soil.

Comparison of the effects of fungal endophyte Gilmaniella sp. and its elicitor on Atractylodes lancea plantlets.

The antimicrobial profile of extracts of a Phormidium-like cyanobacterium changes with phosphate levels.

Effect of formulated root endophytic fungus Piriformospora indica and plant growth promoting rhizobacteria fluorescent pseudomonads R62 and R81 on Vigna mungo.

Diazotrophic diversity in the rhizosphere of two exotic weed plants, Prosopis juliflora and Parthenium hysterophorus.

Genotypic diversity among rhizospheric bacteria of three legumes assessed by cultivation-dependent and cultivation-independent techniques.

Removal of chromium using Rhizobium leguminosarum.

Effect of seed sludge microbial community and activity on the performance of anaerobic reactors during the start-up period.

Effects of wetland degradation on bacterial community in the Zoige Wetland of Qinghai-Tibetan Plateau (China).

Assessment of fungal diversity in deep-sea sediments by multiple primer approach.

Characterization of the RNA polymerase subunit operon from Corynebacterium ammoniagenes.

Genus-specific and phase-dependent effects of nitrate on a sulfate-reducing bacterial community as revealed by dsrB-based DGGE analyses of wastewater reactors.

A new xylanase from Streptomyces megasporus DSM 41476 with high yield of xylobiose.

Evaluation of rhizospheric Pseudomonas and Bacillus as biocontrol tool for Xanthomonas campestris pv campestris.

Mitogenic response and probiotic characteristics of lactic acid bacteria isolated from indigenously pickled vegetables and fermented beverages.

Molecular identification and characterization of clustered regularly interspaced short palindromic repeats (CRISPRs) in a urease-positive thermophilic Campylobacter sp. (UPTC).

Improved synthesis of 2'-deoxyadenosine and 5-methyluridine by Escherichia coli using an auto-induction system.

Hairy root cultures of butterfly pea (Clitoria ternatea L.): Agrobacterium × plant factors influencing transformation.

Abiotic elicitation of gymnemic acid in the suspension cultures of Gymnema sylvestre

An effective and simplified scale-up strategy for acarbose fermentation based on the carbon source control.

The use of FAME analyses to discriminate between different strains of Geotrichum klebahnii with different viabilities.

Phylogenetic study of sponge associated bacteria from the Lakshadweep archipelago and the antimicrobial activities of their secondary metabolites.

Effects of chemical additives and ensiling time on whole plant wheat silage microbial profiles inferred by phenotypic and 16S ribosomal DNA analyses.

Year: 2012 Volume: 28 Issue: 1

Year: 2011 Volume: 27

Year: 2010 Volume: 26

Year: 2007 Volume: 23

Year: 1996 Volume: 12

Year: 1995 Volume: 11

Year: 1994 Volume: 10

Assessment of fungal diversity in deep-sea sediments by multiple primer approach.

Content Provider	World Health Organization (WHO)-Global Index Medicus
Author	Singh, Purnima Raghukumar, Chandralata Verma, Pankaj Shouche, Yogesh
Description	Country affiliation: India Author Affiliation: Singh P ( Council for Scientific and Industrial Research, National Institute of Oceanography, Dona Paula, Goa, 403004, India.)
Abstract	Increasing evidence of the fungal diversity in deep-sea sediments has come from amplification of environmental DNA with fungal specific or eukaryote primer sets. In order to assess the fungal diversity in deep-sea sediments of the Central Indian Basin (CIB) at ~5,000 m depth, we amplified sediment DNA with four different primer sets. These were fungal-specific primer pair ITS1F/ITS4 (internal transcribed spacers), universal 18S rDNA primers NS1/NS2, Euk18S-42F/Euk18S-1492R and Euk18S-555F/Euk18S-1269R. One environmental library was constructed with each of the primer pairs, and 48 clones were sequenced per library. These sequences resulted in 8 fungal Operational Taxonomic Units (OTUs) with ITS and 19 OTUs with 18S rDNA primer sets respectively by taking into account the 2% sequence divergence cut-off for species delineation. These OTUs belonged to 20 distinct fungal genera of the phyla Ascomycota and Basidiomycota. Seven sequences were found to be divergent by 79-97% from the known sequences of the existing database and may be novel. A majority of the sequences clustered with known sequences of the existing taxa. The phylogenetic affiliation of a few fungal sequences with known environmental sequences from marine and hypersaline habitat suggests their autochthonous nature or adaptation to marine habitat. The amplification of sequences belonging to Exobasidiomycetes and Cystobasidiomycetes from deep-sea is being reported for the first time in this study. Amplification of fungal sequences with eukaryotic as well as fungal specific primers indicates that among eukaryotes, fungi appear to be a dominant group in the sampling site of the CIB.
File Format	HTM / HTML
ISSN	09593993
Issue Number	2
Volume Number	28
e-ISSN	15730972
Journal	World Journal of Microbiology and Biotechnology
Language	English
Publisher	Springer
Publisher Date	2012-02-01
Publisher Place	Germany
Access Restriction	One Nation One Subscription (ONOS)
Subject Keyword	Fungi Dna Primers Geologic Sediments Research Support, Non-u.s. Gov't Discipline Microbiology Microbiology Rna, Ribosomal, 18s Classification Genetics Phylogeny Journal Article Discipline Biotechnology
Content Type	Text
Resource Type	Article
Subject	Physiology Medicine Applied Microbiology and Biotechnology Biotechnology

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in

Assessment of fungal diversity in deep-sea sediments by multiple primer approach

Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR-DGGE.

Seasonal and spatial diversity of microbial communities in marine sediments of the South China Sea.

Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials.

Diversity and phylogeny of culturable spore-forming Bacilli isolated from marine sediments.

A molecular phylogeny of thermophilic fungi.

Richness and diversity of bacteria in the Nansha carbonate platform (Core MD05-2896), South China Sea.

Diversity and distributional patterns of ciliates in Guaymas Basin hydrothermal vent sediments.

Microbial diversity in the deep-sea sediments of Iheya North and Iheya Ridge, Okinawa Trough.

Assessment of fungal diversity in deep-sea sediments by multiple primer approach.

Similar Documents

Assessment of fungal diversity in deep-sea sediments by multiple primer approach

Comparison of ITS and 18S rDNA for estimating fungal diversity using PCR-DGGE.

Seasonal and spatial diversity of microbial communities in marine sediments of the South China Sea.

Molecular characterization of multiple xylanase producing thermophilic/thermotolerant fungi isolated from composting materials.

Diversity and phylogeny of culturable spore-forming Bacilli isolated from marine sediments.

A molecular phylogeny of thermophilic fungi.

Richness and diversity of bacteria in the Nansha carbonate platform (Core MD05-2896), South China Sea.

Diversity and distributional patterns of ciliates in Guaymas Basin hydrothermal vent sediments.

Microbial diversity in the deep-sea sediments of Iheya North and Iheya Ridge, Okinawa Trough.

Assessment of fungal diversity in deep-sea sediments by multiple primer approach.